GlyQ

• Description: glycyl-tRNA synthetase (alpha subunit)
  
  

Gene name	glyQ
Synonyms	yqfJ
Essential	yes PubMed
Product	glycyl-tRNA synthetase (alpha subunit)
Function	translation
Gene expression levels in SubtiExpress: glyQ
Interactions involving this protein in SubtInteract: GlyQ
Metabolic function and regulation of this protein in SubtiPathways: glyQ
MW, pI	33 kDa, 4.8
Gene length, protein length	885 bp, 295 aa
Immediate neighbours	glyS, recO
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation, essential genes, most abundant proteins

This gene is a member of the following regulons

T-box

The gene

Basic information

• Locus tag: BSU25270

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU25270

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly) (according to Swiss-Prot)

• Protein family: class-II aminoacyl-tRNA synthetase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions: GlyQ-GlyS

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU25270

• Structure: 1J5W (from Thermotoga maritima, 55% identity, 72% similarity)

• UniProt: P54380

• KEGG entry: [3]

• E.C. number: 6.1.1.14

Expression and regulation

• Operon: glyQ-glyS (according to DBTBS)

• Expression browser: glyQ PubMed

• Sigma factor:

• Regulation:
  • induced by glycine limitation (T-box) PubMed

• Regulatory mechanism:
  • T-box: RNA switch, transcriptional antitermination PubMed

• Additional information:
  • belongs to the 100 most abundant proteins PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 821 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 1704 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 1622 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 1214 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 1611 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: