AroF
Revision as of 13:32, 17 April 2014 by 134.76.70.252 (talk)
- Description: chorismate synthase
Gene name | aroF |
Synonyms | |
Essential | no |
Product | chorismate synthase |
Function | biosynthesis of aromatic amino acids |
Gene expression levels in SubtiExpress: aroF | |
Metabolic function and regulation of this protein in SubtiPathways: aroF | |
MW, pI | 40 kDa, 6.578 |
Gene length, protein length | 1107 bp, 369 aa |
Immediate neighbours | aroB, cheR |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
biosynthesis/ acquisition of amino acids, phosphoproteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU22710
Phenotypes of a mutant
Database entries
- BsubCyc: BSU22710
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: 5-O-(1-carboxyvinyl)-3-phosphoshikimate = chorismate + phosphate (according to Swiss-Prot)
- Protein family: acetokinase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- phosphorylated on Arg-127 PubMed
- Cofactor(s):
- Effectors of protein activity:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- BsubCyc: BSU22710
- Structure: 2O11 (from Mycobacterium tuberculosis h37rv mutant, 42% identity, 58% similarity)
- UniProt: P31104
- KEGG entry: [2]
- E.C. number: 4.2.3.5
Additional information
Expression and regulation
- Operon:
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
- number of protein molecules per cell (minimal medium with glucose and ammonium): 4162 PubMed
- number of protein molecules per cell (complex medium with amino acids, without glucose): 2080 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 1814 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 3069 PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Alexander K W Elsholz, Kürsad Turgay, Stephan Michalik, Bernd Hessling, Katrin Gronau, Dan Oertel, Ulrike Mäder, Jörg Bernhardt, Dörte Becher, Michael Hecker, Ulf Gerth
Global impact of protein arginine phosphorylation on the physiology of Bacillus subtilis.
Proc Natl Acad Sci U S A: 2012, 109(19);7451-6
[PubMed:22517742]
[WorldCat.org]
[DOI]
(I p)
S O Hoch
Mapping of the 5-methyltryptophan resistance locus in Bacillus subtilis.
J Bacteriol: 1974, 117(1);315-7
[PubMed:4202998]
[WorldCat.org]
[DOI]
(P p)