YwjH

• Description: transaldolase
  
  

Gene name	ywjH
Synonyms
Essential	no
Product	transaldolase
Function	pentose phosphate pathway
Gene expression levels in SubtiExpress: ywjH
Metabolic function and regulation of this protein in SubtiPathways: ywjH
MW, pI	22 kDa, 5.876
Gene length, protein length	636 bp, 212 aa
Immediate neighbours	murAB, fbaA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

carbon core metabolism, phosphoproteins, most abundant proteins

The gene

Basic information

• Locus tag: BSU37110

Database entries

• BsubCyc: BSU37110

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-erythrose 4-phosphate + D-fructose 6-phosphate (according to Swiss-Prot)

• Protein family: Type 3B subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylation on Ser-39 PubMed, in vitro phosphorylated by PrkC PubMed

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • forms decamers (dimer of pentamers) PubMed

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU37110

• Structure: 3R8R PubMed

• UniProt: P19669

• KEGG entry: [3]

• E.C. number: 2.2.1.2

Expression and regulation

• Operon: fbaA-ywjH PubMed

• Expression browser: ywjH PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • belongs to the 100 most abundant proteins PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 9591 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 25813 PubMed

Biological materials

• Mutant:

• Expression vector:
  • for expression, purification in E. coli with N-terminal Strep-tag, in pGP172: pGP819, available in Stülke lab
  • pGP1790 (expression of ywjH in B. subtilis, in pGP1389), available in Stülke lab
  • GP1409 (ywjH-Strep (spc)) & GP1411 (ywjH-Strep (cat)), purification from B. subtilis, for SPINE, available in Stülke lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab

• Antibody:

• FLAG-tag construct: GP1407 (spc, based on pGP1331), available in the Stülke lab

References

Anne K Samland, Shiromi Baier, Melanie Schürmann, Tomoyuki Inoue, Sabine Huf, Gunter Schneider, Georg A Sprenger, Tatyana Sandalova
Conservation of structure and mechanism within the transaldolase enzyme family.
FEBS J: 2012, 279(5);766-78
[PubMed:22212631] [WorldCat.org] [DOI] (I p)

Anja Lehwess-Litzmann, Piotr Neumann, Christoph Parthier, Stefan Lüdtke, Ralph Golbik, Ralf Ficner, Kai Tittmann
Twisted Schiff base intermediates and substrate locale revise transaldolase mechanism.
Nat Chem Biol: 2011, 7(10);678-84
[PubMed:21857661] [WorldCat.org] [DOI] (I e)

Nico Pietack, Dörte Becher, Sebastian R Schmidl, Milton H Saier, Michael Hecker, Fabian M Commichau, Jörg Stülke
In vitro phosphorylation of key metabolic enzymes from Bacillus subtilis: PrkC phosphorylates enzymes from different branches of basic metabolism.
J Mol Microbiol Biotechnol: 2010, 18(3);129-40
[PubMed:20389117] [WorldCat.org] [DOI] (I p)

Christine Eymann, Dörte Becher, Jörg Bernhardt, Katrin Gronau, Anja Klutzny, Michael Hecker
Dynamics of protein phosphorylation on Ser/Thr/Tyr in Bacillus subtilis.
Proteomics: 2007, 7(19);3509-26
[PubMed:17726680] [WorldCat.org] [DOI] (P p)

Boris Macek, Ivan Mijakovic, Jesper V Olsen, Florian Gnad, Chanchal Kumar, Peter R Jensen, Matthias Mann
The serine/threonine/tyrosine phosphoproteome of the model bacterium Bacillus subtilis.
Mol Cell Proteomics: 2007, 6(4);697-707
[PubMed:17218307] [WorldCat.org] [DOI] (P p)

Alain Lévine, Françoise Vannier, Cédric Absalon, Lauriane Kuhn, Peter Jackson, Elaine Scrivener, Valérie Labas, Joëlle Vinh, Patrick Courtney, Jérôme Garin, Simone J Séror
Analysis of the dynamic Bacillus subtilis Ser/Thr/Tyr phosphoproteome implicated in a wide variety of cellular processes.
Proteomics: 2006, 6(7);2157-73
[PubMed:16493705] [WorldCat.org] [DOI] (P p)

Christine Eymann, Annette Dreisbach, Dirk Albrecht, Jörg Bernhardt, Dörte Becher, Sandy Gentner, Le Thi Tam, Knut Büttner, Gerrit Buurman, Christian Scharf, Simone Venz, Uwe Völker, Michael Hecker
A comprehensive proteome map of growing Bacillus subtilis cells.
Proteomics: 2004, 4(10);2849-76
[PubMed:15378759] [WorldCat.org] [DOI] (P p)

H Ludwig, G Homuth, M Schmalisch, F M Dyka, M Hecker, J Stülke
Transcription of glycolytic genes and operons in Bacillus subtilis: evidence for the presence of multiple levels of control of the gapA operon.
Mol Microbiol: 2001, 41(2);409-22
[PubMed:11489127] [WorldCat.org] [DOI] (P p)