RpsD

• Description: ribosomal protein
  
  

Gene name	rpsD
Synonyms
Essential	yes PubMed
Product	ribosomal protein S4 (BS4)
Function	translation
Gene expression levels in SubtiExpress: rpsD
Interactions involving this protein in SubtInteract: RpsD
MW, pI	22 kDa, 10.243
Gene length, protein length	600 bp, 200 aa
Immediate neighbours	dgcP, tyrS
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation, essential genes, universally conserved proteins

This gene is a member of the following regulons

stringent response

The gene

Basic information

• Locus tag: BSU29660

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU29660

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: ribosomal protein S4P family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU29660

• Structure: 1C06 (Geobacillus stearothermophilus)

• UniProt: P21466

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: rpsD PubMed

• Expression browser: rpsD PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • RelA dependent downregulation (Class I) during stringent response PubMed
  • autorepression of rpsD expression upon binding of excess RpsD to a RNA switch in the untranslated region of the rpsD mRNA PubMed

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 8008 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 42285 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Kaila Deiorio-Haggar, Jon Anthony, Michelle M Meyer
RNA structures regulating ribosomal protein biosynthesis in bacilli.
RNA Biol: 2013, 10(7);1180-4
[PubMed:23611891] [WorldCat.org] [DOI] (I p)

Genki Akanuma, Hideaki Nanamiya, Yousuke Natori, Koichi Yano, Shota Suzuki, Shuya Omata, Morio Ishizuka, Yasuhiko Sekine, Fujio Kawamura
Inactivation of ribosomal protein genes in Bacillus subtilis reveals importance of each ribosomal protein for cell proliferation and cell differentiation.
J Bacteriol: 2012, 194(22);6282-91
[PubMed:23002217] [WorldCat.org] [DOI] (I p)

Matthew A Lauber, William E Running, James P Reilly
B. subtilis ribosomal proteins: structural homology and post-translational modifications.
J Proteome Res: 2009, 8(9);4193-206
[PubMed:19653700] [WorldCat.org] [DOI] (P p)

T Inaoka, K Kasai, K Ochi
Construction of an in vivo nonsense readthrough assay system and functional analysis of ribosomal proteins S12, S4, and S5 in Bacillus subtilis.
J Bacteriol: 2001, 183(17);4958-63
[PubMed:11489846] [WorldCat.org] [DOI] (P p)

F J Grundy, T M Henkin
Characterization of the Bacillus subtilis rpsD regulatory target site.
J Bacteriol: 1992, 174(21);6763-70
[PubMed:1400226] [WorldCat.org] [DOI] (P p)

F J Grundy, T M Henkin
The rpsD gene, encoding ribosomal protein S4, is autogenously regulated in Bacillus subtilis.
J Bacteriol: 1991, 173(15);4595-602
[PubMed:1906866] [WorldCat.org] [DOI] (P p)

T M Henkin, G H Chambliss, F J Grundy
Bacillus subtilis mutants with alterations in ribosomal protein S4.
J Bacteriol: 1990, 172(11);6380-5
[PubMed:2121712] [WorldCat.org] [DOI] (P p)

F J Grundy, T M Henkin
Cloning and analysis of the Bacillus subtilis rpsD gene, encoding ribosomal protein S4.
J Bacteriol: 1990, 172(11);6372-9
[PubMed:1699930] [WorldCat.org] [DOI] (P p)

T M Henkin, G H Chambliss
Genetic mapping of a mutation causing an alteration in Bacillus subtilis ribosomal protein S4.
Mol Gen Genet: 1984, 193(2);364-9
[PubMed:6420647] [WorldCat.org] [DOI] (P p)