RnhC
- Description: RNase HIII, endoribonuclease
| Gene name | rnhC |
| Synonyms | ysgB |
| Essential | no |
| Product | Mg2+-dependent RNase HIII |
| Function | endonucleolytic cleavage of RNA in RNA-DNA hybrid molecules |
| Gene expression levels in SubtiExpress: rnhC | |
| MW, pI | 33 kDa, 10.07 |
| Gene length, protein length | 939 bp, 313 aa |
| Immediate neighbours | zapA, pheT |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
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Expression at a glance PubMed
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Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU28620
Phenotypes of a mutant
Database entries
- BsubCyc: BSU28620
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Endonucleolytic cleavage to 5'-phosphomonoester (according to Swiss-Prot)
- Protein family: RnhC subfamily (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- RnhC interacts with the RNA polymerase PubMed
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- BsubCyc: BSU28620
- Structure: 2D0B (complex with Mg2+, Geobacillus stearothermophilus , 47% identity), 2D0A (Geobacillus stearothermophilus, 47% identity)
- UniProt: P94541
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: BP430 (ΔrnhC::cat) available in Fabian Commichau's lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Karolina A Majorek, Stanislaw Dunin-Horkawicz, Kamil Steczkiewicz, Anna Muszewska, Marcin Nowotny, Krzysztof Ginalski, Janusz M Bujnicki
The RNase H-like superfamily: new members, comparative structural analysis and evolutionary classification.
Nucleic Acids Res: 2014, 42(7);4160-79
[PubMed:24464998]
[WorldCat.org]
[DOI]
(I p)
Takashi Tadokoro, Shigenori Kanaya
Ribonuclease H: molecular diversities, substrate binding domains, and catalytic mechanism of the prokaryotic enzymes.
FEBS J: 2009, 276(6);1482-93
[PubMed:19228197]
[WorldCat.org]
[DOI]
(I p)
Original publications
Nina Y Yao, Jeremy W Schroeder, Olga Yurieva, Lyle A Simmons, Mike E O'Donnell
Cost of rNTP/dNTP pool imbalance at the replication fork.
Proc Natl Acad Sci U S A: 2013, 110(32);12942-7
[PubMed:23882084]
[WorldCat.org]
[DOI]
(I p)
Olivier Delumeau, François Lecointe, Jan Muntel, Alain Guillot, Eric Guédon, Véronique Monnet, Michael Hecker, Dörte Becher, Patrice Polard, Philippe Noirot
The dynamic protein partnership of RNA polymerase in Bacillus subtilis.
Proteomics: 2011, 11(15);2992-3001
[PubMed:21710567]
[WorldCat.org]
[DOI]
(I p)
Sanae Fukushima, Mitsuhiro Itaya, Hiroaki Kato, Naotake Ogasawara, Hirofumi Yoshikawa
Reassessment of the in vivo functions of DNA polymerase I and RNase H in bacterial cell growth.
J Bacteriol: 2007, 189(23);8575-83
[PubMed:17905985]
[WorldCat.org]
[DOI]
(I p)
M Itaya, A Omori, S Kanaya, R J Crouch, T Tanaka, K Kondo
Isolation of RNase H genes that are essential for growth of Bacillus subtilis 168.
J Bacteriol: 1999, 181(7);2118-23
[PubMed:10094689]
[WorldCat.org]
[DOI]
(P p)
N Ohtani, M Haruki, M Morikawa, R J Crouch, M Itaya, S Kanaya
Identification of the genes encoding Mn2+-dependent RNase HII and Mg2+-dependent RNase HIII from Bacillus subtilis: classification of RNases H into three families.
Biochemistry: 1999, 38(2);605-18
[PubMed:9888800]
[WorldCat.org]
[DOI]
(P p)
A Wipat, N Carter, S C Brignell, B J Guy, K Piper, J Sanders, P T Emmerson, C R Harwood
The dnaB-pheA (256 degrees-240 degrees) region of the Bacillus subtilis chromosome containing genes responsible for stress responses, the utilization of plant cell walls and primary metabolism.
Microbiology (Reading): 1996, 142 ( Pt 11);3067-78
[PubMed:8969504]
[WorldCat.org]
[DOI]
(P p)
