HemB

• Description: porphobilinogen synthase
  
  

Gene name	hemB
Synonyms
Essential	no
Product	porphobilinogen synthase
Function	heme biosynthesis
Gene expression levels in SubtiExpress: hemB
Metabolic function and regulation of this protein in SubtiPathways: HemB
MW, pI	36 kDa, 4.98
Gene length, protein length	972 bp, 324 aa
Immediate neighbours	hemL, hemD
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis of cofactors, phosphoproteins

This gene is a member of the following regulons

PerR regulon

The gene

Basic information

• Locus tag: BSU28130

Database entries

• BsubCyc: BSU28130

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 2 5-aminolevulinate = porphobilinogen + 2 H₂O (according to Swiss-Prot)

• Protein family: ALADH family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • phosphorylated on Arg-217 PubMed

• Cofactor(s): zinc

• Effectors of protein activity:

• Interactions:

• Localization:
  • cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU28130

• Structure:

• UniProt: P30950

• KEGG entry: [3]

• E.C. number: 4.2.1.24

Expression and regulation

• Operon: hemA-hemX-hemC-hemD-hemB-hemL PubMed

• Expression browser: hemB PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by hydrogen peroxide (PerR) PubMed

• Regulatory mechanism:
  • PerR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: