Tgt

• Description: tRNA-guanine transglycosylase
  
  

Gene name	tgt
Synonyms
Essential	no
Product	tRNA-guanine transglycosylase
Function	tRNA modification
Gene expression levels in SubtiExpress: tgt
MW, pI	43 kDa, 6.149
Gene length, protein length	1143 bp, 381 aa
Immediate neighbours	yrbF, queA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation, membrane proteins

The gene

Basic information

• Locus tag: BSU27710

Database entries

• BsubCyc: BSU27710

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: [tRNA]-guanine + queuine = [tRNA]-queuine + guanine (according to Swiss-Prot)

• Protein family: queuine tRNA-ribosyltransferase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• BsubCyc: BSU27710

• Structure:

• UniProt: O32053

• KEGG entry: [2]

• E.C. number: 2.4.2.29

Expression and regulation

• Operon:

• Expression browser: tgt PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 258 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 1005 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: