TxpA

• Description: toxic peptide
  
  

Gene name	txpA
Synonyms	yqcR, yqdB
Essential	no
Product	toxic peptide
Function	toxin
Gene expression levels in SubtiExpress: txpA
MW, pI	6 kDa, 8.139
Gene length, protein length	177 bp, 59 aa
Immediate neighbours	yqbN/2, ratA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

toxins, antitoxins and immunity against toxins, Skin element, membrane proteins

This gene is a member of the following regulons

ratA regulon

The gene

Basic information

• Locus tag: BSU26050

Database entries

• BsubCyc: BSU26050

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane

Database entries

• BsubCyc: BSU26050

• Structure:

• UniProt: P54373

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: txpA PubMed

• Expression browser: txpA PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:
  • expression is prevented by the ratA antisense RNA PubMed
  • the txpA mRNA-RatA RNA duplex is degraded by RNase III PubMed

• Additional information:
  • next to yonT, the txpA mRNA has the strongest Shine-Dalgarno region in B. subtilis PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Richard Losick, Harvard University, Cambridge, USA Homepage

References

Original publications

Sylvain Durand, Laetitia Gilet, Ciarán Condon
The essential function of B. subtilis RNase III is to silence foreign toxin genes.
PLoS Genet: 2012, 8(12);e1003181
[PubMed:23300471] [WorldCat.org] [DOI] (I p)

Roula Daou-Chabo, Nathalie Mathy, Lionel Bénard, Ciarán Condon
Ribosomes initiating translation of the hbs mRNA protect it from 5'-to-3' exoribonucleolytic degradation by RNase J1.
Mol Microbiol: 2009, 71(6);1538-50
[PubMed:19210617] [WorldCat.org] [DOI] (I p)

Jessica M Silvaggi, John B Perkins, Richard Losick
Small untranslated RNA antitoxin in Bacillus subtilis.
J Bacteriol: 2005, 187(19);6641-50
[PubMed:16166525] [WorldCat.org] [DOI] (P p)