RpoY

• Description: epsilon subunit of RNA polymerase
  
  

Gene name	ykzG
Synonyms
Essential	no
Product	epsilon subunit of RNA polymerase
Function	control of RNA polymerase activity
Gene expression levels in SubtiExpress: ykzG
MW, pI	8 kDa, 4.605
Gene length, protein length	207 bp, 69 aa
Immediate neighbours	rnjA, ykrA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transcription

The gene

Basic information

• Locus tag: BSU14540

Database entries

• BsubCyc: BSU14540

• DBTBS entry: no entry

• SubtiList entry: [1]

Additional information

The protein is sometimes annotated as omega1 subunit of RNA polymerase, however, there is no experimental evidence in support of this ("it is unrelated in sequence to ω/Rpb6 from the eubacteria, archaea and eukaryotes, unpublished data suggests it does not bind at the same site as ω/Rpb6 subunits bind their cognate RNAPs, and therefore it is unlikely to function as a true ω subunit") PubMed

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: UPF0356 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU14540

• Structure:

• UniProt: O31718

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: ykzG-rnjA PubMed

• Expression browser: ykzG PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:
  • translation of YkzG and RnjA is coupled, and this coupling is required for efficient expression of RNase J1 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 443 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion: pGP420 (in pAC7), available in Stülke lab

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Ailar Jamalli, Agnès Hébert, Léna Zig, Harald Putzer
Control of expression of the RNases J1 and J2 in Bacillus subtilis.
J Bacteriol: 2014, 196(2);318-24
[PubMed:24187087] [WorldCat.org] [DOI] (I p)

Geoff P Doherty, Mark J Fogg, Anthony J Wilkinson, Peter J Lewis
Small subunits of RNA polymerase: localization, levels and implications for core enzyme composition.
Microbiology (Reading): 2010, 156(Pt 12);3532-3543
[PubMed:20724389] [WorldCat.org] [DOI] (I p)