AroD

• Description: shikimate dehydrogenase
  
  

Gene name	aroD
Synonyms
Essential	no
Product	shikimate dehydrogenase
Function	biosynthesis of aromatic amino acids
Gene expression levels in SubtiExpress: aroD
Metabolic function and regulation of this protein in SubtiPathways: aroD
MW, pI	30 kDa, 6.077
Gene length, protein length	840 bp, 280 aa
Immediate neighbours	yqeI, yqeH
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

The gene

Basic information

• Locus tag: BSU25660

Database entries

• BsubCyc: BSU25660

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Shikimate + NADP⁺ = 3-dehydroshikimate + NADPH (according to Swiss-Prot)

• Protein family: shikimate dehydrogenase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU25660

• Structure: 2HK8 (from Aquifex aeolicus, 42% identity, 61% similarity) PubMed

• UniProt: P54374

• KEGG entry: [2]

• E.C. number: 1.1.1.25

Expression and regulation

• Operon:

• Expression browser: aroD PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Dong-Feng Liu, Guo-Min Ai, Qing-Xiang Zheng, Chang Liu, Cheng-Ying Jiang, Li-Xia Liu, Bo Zhang, Yi-Ming Liu, Chen Yang, Shuang-Jiang Liu
Metabolic flux responses to genetic modification for shikimic acid production by Bacillus subtilis strains.
Microb Cell Fact: 2014, 13(1);40
[PubMed:24628944] [WorldCat.org] [DOI] (I e)

J Bergsma, K E Meihuizen, W Van Oeveren, W N Konings
Restoration of NADH oxidation with menaquinones and menaquinone analogues in membrane vesicles from the menaquinone-deficient Bacillus subtilis aroD.
Eur J Biochem: 1982, 125(3);651-7
[PubMed:6811271] [WorldCat.org] [DOI] (P p)