MotB

• Description: H+-coupled MotA-MotB flagellar stator
  
  

Gene name	motB
Synonyms	mot
Essential	no
Product	motility protein (flagellar motor rotation)
Function	motility and chemotaxis
Gene expression levels in SubtiExpress: motB
Interactions involving this protein in SubtInteract: MotB
MW, pI	29 kDa, 6.652
Gene length, protein length	783 bp, 261 aa
Immediate neighbours	mhqR, motA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

motility and chemotaxis, membrane proteins

This gene is a member of the following regulons

SigD regulon

The gene

Basic information

• Locus tag: BSU13680

Phenotypes of a mutant

• loss of swimming and swarming motility PubMed
• mucoid phenotype due to the DegU-P activated overexpression of the capB-capC-capA-capE operon and resulting overproduction of poly-gamma-glutamate PubMed

Database entries

• BsubCyc: BSU13680

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: OmpA-like domain (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • protonation of Asp-24 is essential for torque generation PubMed

• Cofactors:

• Effectors of protein activity:

• Interactions: MotA-MotB

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• BsubCyc: BSU13680

• Structure:

• UniProt: P28612

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: motA-motB PubMed

• Expression browser: motB PubMed

• Sigma factor: SigD PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: available in Nicola Stanley-Wall's lab PubMed

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Jia Mun Chan, Sarah B Guttenplan, Daniel B Kearns
Defects in the flagellar motor increase synthesis of poly-γ-glutamate in Bacillus subtilis.
J Bacteriol: 2014, 196(4);740-53
[PubMed:24296669] [WorldCat.org] [DOI] (I p)

Lynne S Cairns, Victoria L Marlow, Emma Bissett, Adam Ostrowski, Nicola R Stanley-Wall
A mechanical signal transmitted by the flagellum controls signalling in Bacillus subtilis.
Mol Microbiol: 2013, 90(1);6-21
[PubMed:23888912] [WorldCat.org] [DOI] (I p)

J Zhou, L L Sharp, H L Tang, S A Lloyd, S Billings, T F Braun, D F Blair
Function of protonatable residues in the flagellar motor of Escherichia coli: a critical role for Asp 32 of MotB.
J Bacteriol: 1998, 180(10);2729-35
[PubMed:9573160] [WorldCat.org] [DOI] (P p)

M Z Gilman, M J Chamberlin
Developmental and genetic regulation of Bacillus subtilis genes transcribed by sigma 28-RNA polymerase.
Cell: 1983, 35(1);285-93
[PubMed:6313226] [WorldCat.org] [DOI] (P p)