Nap

• Description: carboxylesterase NA
  
  

Gene name	nap
Synonyms	cesA
Essential	no
Product	carboxylesterase NA
Function	lipid degradation
Gene expression levels in SubtiExpress: nap
MW, pI	33 kDa, 5.798
Gene length, protein length	900 bp, 300 aa
Immediate neighbours	ydfJ, ydfK
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of lipids

The gene

Basic information

• Locus tag: BSU05440

Database entries

• BsubCyc: BSU05440

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: A carboxylic ester + H₂O = an alcohol + a carboxylate (according to Swiss-Prot)

• Protein family: AB hydrolase superfamily (according to Swiss-Prot)

• Paralogous protein(s): YbfK

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU05440

• Structure: 2R11

• UniProt: P96688

• KEGG entry: [3]

• E.C. number: 3.1.1.1 3.1.1.1]

Expression and regulation

• Operon: nap PubMed

• Expression browser: nap PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Henriëtte J Rozeboom, Luis F Godinho, Marco Nardini, Wim J Quax, Bauke W Dijkstra
Crystal structures of two Bacillus carboxylesterases with different enantioselectivities.
Biochim Biophys Acta: 2014, 1844(3);567-75
[PubMed:24418394] [WorldCat.org] [DOI] (P p)

Luis F Godinho, Carlos R Reis, Henriëtte J Rozeboom, Frank J Dekker, Bauke W Dijkstra, Gerrit J Poelarends, Wim J Quax
Enhancement of the enantioselectivity of carboxylesterase A by structure-based mutagenesis.
J Biotechnol: 2012, 158(1-2);36-43
[PubMed:22248594] [WorldCat.org] [DOI] (I p)

Ken-ichi Yoshida, Hirotake Yamaguchi, Masaki Kinehara, Yo-hei Ohki, Yoshiko Nakaura, Yasutaro Fujita
Identification of additional TnrA-regulated genes of Bacillus subtilis associated with a TnrA box.
Mol Microbiol: 2003, 49(1);157-65
[PubMed:12823818] [WorldCat.org] [DOI] (P p)