MinJ

• Description: topological determinant of cell division, part of the Min system (with DivIVA, MinC, MinD), Noc and the Min system ensure the efficient utilization of the division site at midcell in by ensuring Z ring placement
  
  

Gene name	minJ
Synonyms	yvjD, swrAB
Essential	no
Product	topological determinant of cell division
Function	cell division site selection
Gene expression levels in SubtiExpress: minJ
Interactions involving this protein in SubtInteract: MinJ
MW, pI	43 kDa, 9.057
Gene length, protein length	1191 bp, 397 aa
Immediate neighbours	yvkA, swrAA/1
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell division, membrane proteins

The gene

Basic information

• Locus tag: BSU35220

Database entries

• BsubCyc: BSU35220

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: The Min system prevents minicell formation adjacent to recently completed division sites by promoting the disassembly of the cytokinetic ring, thereby ensuring that cell division occurs only once per cell cycle PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • (MinC-MinD)-MinJ-DivIVA PubMed, MinJ binds to the N-terminal lipid-binding domain of DivIVA PubMed

• Localization:
  • forms rings at the division septum PubMed
  • cell membrane PubMed

Database entries

• BsubCyc: BSU35220

• Structure:

• UniProt: O34375

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: swrAA-minJ PubMed

• Expression browser: minJ PubMed

• Sigma factor: SigD PubMed, SigA PubMed

• Regulation:

• Regulatory mechanism:
  • DegU-P: transcription activation (SigA promoter) PubMed
• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Daniel Kearns, Indiana University, Bloomington, USA, homepage

Marc Bramkamp, University of Cologne, Cologne, Germany homepage

References

Reviews

Imrich Barák
Open questions about the function and evolution of bacterial Min systems.
Front Microbiol: 2013, 4;378
[PubMed:24367361] [WorldCat.org] [DOI] (P e)

Marc Bramkamp, Suey van Baarle
Division site selection in rod-shaped bacteria.
Curr Opin Microbiol: 2009, 12(6);683-8
[PubMed:19884039] [WorldCat.org] [DOI] (I p)

Original Publications

Suey van Baarle, Ilkay Nazli Celik, Karan Gautam Kaval, Marc Bramkamp, Leendert W Hamoen, Sven Halbedel
Protein-protein interaction domains of Bacillus subtilis DivIVA.
J Bacteriol: 2013, 195(5);1012-21
[PubMed:23264578] [WorldCat.org] [DOI] (I p)

Christopher D A Rodrigues, Elizabeth J Harry
The Min system and nucleoid occlusion are not required for identifying the division site in Bacillus subtilis but ensure its efficient utilization.
PLoS Genet: 2012, 8(3);e1002561
[PubMed:22457634] [WorldCat.org] [DOI] (I p)

Prahathees Eswaramoorthy, Marcella L Erb, James A Gregory, Jared Silverman, Kit Pogliano, Joe Pogliano, Kumaran S Ramamurthi
Cellular architecture mediates DivIVA ultrastructure and regulates min activity in Bacillus subtilis.
mBio: 2011, 2(6);
[PubMed:22108385] [WorldCat.org] [DOI] (I e)

Suey van Baarle, Marc Bramkamp
The MinCDJ system in Bacillus subtilis prevents minicell formation by promoting divisome disassembly.
PLoS One: 2010, 5(3);e9850
[PubMed:20352045] [WorldCat.org] [DOI] (I e)

Marc Bramkamp, Robyn Emmins, Louise Weston, Catriona Donovan, Richard A Daniel, Jeff Errington
A novel component of the division-site selection system of Bacillus subtilis and a new mode of action for the division inhibitor MinCD.
Mol Microbiol: 2008, 70(6);1556-69
[PubMed:19019154] [WorldCat.org] [DOI] (I p)

Joyce E Patrick, Daniel B Kearns
MinJ (YvjD) is a topological determinant of cell division in Bacillus subtilis.
Mol Microbiol: 2008, 70(5);1166-79
[PubMed:18976281] [WorldCat.org] [DOI] (I p)

Cinzia Calvio, Cecilia Osera, Giuseppe Amati, Alessandro Galizzi
Autoregulation of swrAA and motility in Bacillus subtilis.
J Bacteriol: 2008, 190(16);5720-8
[PubMed:18567663] [WorldCat.org] [DOI] (I p)

Cinzia Calvio, Francesco Celandroni, Emilia Ghelardi, Giuseppe Amati, Sara Salvetti, Fabrizio Ceciliani, Alessandro Galizzi, Sonia Senesi
Swarming differentiation and swimming motility in Bacillus subtilis are controlled by swrA, a newly identified dicistronic operon.
J Bacteriol: 2005, 187(15);5356-66
[PubMed:16030230] [WorldCat.org] [DOI] (P p)