GabR

• Description: transcription activator of gabT-gabD, repressor of gabR (MocR/ GabR family)
  
  

Gene name	gabR
Synonyms	ycnF
Essential	no
Product	transcription regulator
Function	regulation of gamma-amino butyric acid utilization
Gene expression levels in SubtiExpress: gabR
MW, pI	54 kDa, 6.709
Gene length, protein length	1437 bp, 479 aa
Immediate neighbours	yczG, gabT
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of amino acids, transcription factors and their control

This gene is a member of the following regulons

GabR regulon

The GabR regulon:

• gabT-gabD, gabR

The gene

Basic information

• Locus tag: BSU03890

Database entries

• BsubCyc: BSU03890

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • transcription activation of the gabT-gabD operon in the presence of GABA and PLP, transcription repression of gabR PubMed

• Protein family: MocR/ GabR family PubMed

• Paralogous protein(s): none, but there are 7 members of the MocR/ GabR family in B. subtilis

Extended information on the protein

• Kinetic information:

• Domains:
  • N-terminal DNA-binding helix-turn-helix motif (corresponding to domains of the GntR family) PubMed
  • C-terminal domain is homologous to PLP-binding large domain of aminotransferases PubMed

• Modification:

• Cofactors: gamma-aminobutyric acid, PLP PubMed

• Effectors of protein activity:
  • activation of gabT-gabD expression is triggered by gamma-amino butyrate and pyridoxalphosphate PubMed

• Interactions:
  • forms head-to-tail domain-swapped dimers PubMed

• Localization:

Database entries

• BsubCyc: BSU03890

• Structure:
  • 4MGR (the apo-protein) PubMed
  • 4N0B (the complex with PLP) PubMed

• UniProt: P94426

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: gabR (according to DBTBS)

• Expression browser: gabR PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:
  • GabR: negative autoregulation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Linc Sonenshein, Tufts University, Boston, MA, USA Homepage

References

Raji Edayathumangalam, Rui Wu, Roman Garcia, Yuguang Wang, Wei Wang, Cheryl A Kreinbring, Alicia Bach, Jingling Liao, Todd A Stone, Thomas C Terwilliger, Quyen Q Hoang, Boris R Belitsky, Gregory A Petsko, Dagmar Ringe, Dali Liu
Crystal structure of Bacillus subtilis GabR, an autorepressor and transcriptional activator of gabT.
Proc Natl Acad Sci U S A: 2013, 110(44);17820-5
[PubMed:24127574] [WorldCat.org] [DOI] (I p)

E Bramucci, T Milano, S Pascarella
Genomic distribution and heterogeneity of MocR-like transcriptional factors containing a domain belonging to the superfamily of the pyridoxal-5'-phosphate dependent enzymes of fold type I.
Biochem Biophys Res Commun: 2011, 415(1);88-93
[PubMed:22020104] [WorldCat.org] [DOI] (I p)

Boris R Belitsky
Bacillus subtilis GabR, a protein with DNA-binding and aminotransferase domains, is a PLP-dependent transcriptional regulator.
J Mol Biol: 2004, 340(4);655-64
[PubMed:15223311] [WorldCat.org] [DOI] (P p)

Boris R Belitsky, Abraham L Sonenshein
GabR, a member of a novel protein family, regulates the utilization of gamma-aminobutyrate in Bacillus subtilis.
Mol Microbiol: 2002, 45(2);569-83
[PubMed:12123465] [WorldCat.org] [DOI] (P p)

Sébastien Rigali, Adeline Derouaux, Fabrizio Giannotta, Jean Dusart
Subdivision of the helix-turn-helix GntR family of bacterial regulators in the FadR, HutC, MocR, and YtrA subfamilies.
J Biol Chem: 2002, 277(15);12507-15
[PubMed:11756427] [WorldCat.org] [DOI] (P p)