SacP

• Description: trigger enzyme: sucrose-specific phosphotransferase system, EIIBC component of the PTS
  
  

Gene name	sacP
Synonyms	ipa-49d
Essential	no
Product	trigger enzyme: sucrose-specific phosphotransferase system, EIIBC component
Function	sucrose uptake and phosphorylation, control of SacT activity
Gene expression levels in SubtiExpress: sacP
Interactions involving this protein in SubtInteract: SacP
Metabolic function and regulation of this protein in SubtiPathways: sacP
MW, pI	49 kDa, 7.026
Gene length, protein length	1383 bp, 461 aa
Immediate neighbours	sacA, ywcJ
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

phosphotransferase systems, utilization of specific carbon sources, transcription factors and their control, trigger enzyme, membrane proteins, phosphoproteins

This gene is a member of the following regulons

CcpA regulon, SacT regulon

The gene

Basic information

• Locus tag: BSU38050

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate (according to Swiss-Prot)

• Protein family: PTS permease, sucrose permease (Scr) family PubMed

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P05306

• KEGG entry: [3]

• E.C. number: 2.7.1.69

Expression and regulation

• Operon: sacP-sacA-ywdA PubMed

• Expression browser: sacP PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by sucrose (SacT) PubMed
  • repressed by glucose (CcpA)

• Regulatory mechanism:
  • SacT: binding to a RNA switch results in transcription antitermination PubMed
  • CcpA: transcription repression

• Additional information:

Biological materials

• Mutant:

• Expression vector:
  • for expression, purification of the EIIB domain in E. coli with N-terminal His-tag, in pWH844: pGP429, available in Stülke lab
  • for expression, purification of the EIIB domain in E. coli with N-terminal His-tag and thrombin cleavage site, in pGP570: pGP436, available in Stülke lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: