YueB

• Description: membrane protein, part of the type VII protein secretion system YukD-YukC-YukB-YueB-YueC, receptor for phage SPPI
  
  

Gene name	yueB
Synonyms
Essential	no
Product	part of the type VII protein secretion system, receptor for phage SPPI
Function	export of YukE
Gene expression levels in SubtiExpress: yueB
MW, pI	119 kDa, 4.302
Gene length, protein length	3228 bp, 1076 aa
Immediate neighbours	yueC, yukB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

phage-related functions, protein secretion, membrane proteins

This gene is a member of the following regulons

DegU regulon

The gene

Basic information

• Locus tag: BSU31860

Phenotypes of a mutant

• resistance to phage SPPI PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • receptor for phage SPP1 PubMed
  • required for depolarization of the cytoplasmic membrane upon binding of phage SPP1 PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • YueB is a member of a suspected group of hubs proteins that were suggested to be involved in a large number of interactions PubMed

• Localization:
  • cell membrane PubMed, localizes preferentially to the cell poles PubMed
  • exposed to the surface PubMed
  • cytoplasm (homogeneous) PubMed

Database entries

• Structure:

• UniProt: O32101

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: yukE-yukD-yukC-yukB-yueB-yueC PubMed

• Expression browser: yueB PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • not expressed in the lab strain 168, due to lack of DegU-P PubMed
  • expressed in the stationary phase PubMed

• Regulatory mechanism:
  • DegU-P: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Roxane Simeone, Daria Bottai, Roland Brosch
ESX/type VII secretion systems and their role in host-pathogen interaction.
Curr Opin Microbiol: 2009, 12(1);4-10
[PubMed:19155186] [WorldCat.org] [DOI] (I p)

Mark J Pallen
The ESAT-6/WXG100 superfamily -- and a new Gram-positive secretion system?
Trends Microbiol: 2002, 10(5);209-12
[PubMed:11973144] [WorldCat.org] [DOI] (P p)

Original publications

Catarina Baptista, Hugo Condessa Barreto, Carlos São-José
High levels of DegU-P activate an Esat-6-like secretion system in Bacillus subtilis.
PLoS One: 2013, 8(7);e67840
[PubMed:23861817] [WorldCat.org] [DOI] (I e)

Pierre Nicolas, Ulrike Mäder, Etienne Dervyn, Tatiana Rochat, Aurélie Leduc, Nathalie Pigeonneau, Elena Bidnenko, Elodie Marchadier, Mark Hoebeke, Stéphane Aymerich, Dörte Becher, Paola Bisicchia, Eric Botella, Olivier Delumeau, Geoff Doherty, Emma L Denham, Mark J Fogg, Vincent Fromion, Anne Goelzer, Annette Hansen, Elisabeth Härtig, Colin R Harwood, Georg Homuth, Hanne Jarmer, Matthieu Jules, Edda Klipp, Ludovic Le Chat, François Lecointe, Peter Lewis, Wolfram Liebermeister, Anika March, Ruben A T Mars, Priyanka Nannapaneni, David Noone, Susanne Pohl, Bernd Rinn, Frank Rügheimer, Praveen K Sappa, Franck Samson, Marc Schaffer, Benno Schwikowski, Leif Steil, Jörg Stülke, Thomas Wiegert, Kevin M Devine, Anthony J Wilkinson, Jan Maarten van Dijl, Michael Hecker, Uwe Völker, Philippe Bessières, Philippe Noirot
Condition-dependent transcriptome reveals high-level regulatory architecture in Bacillus subtilis.
Science: 2012, 335(6072);1103-6
[PubMed:22383849] [WorldCat.org] [DOI] (I p)

Inês Vinga, Catarina Baptista, Isabelle Auzat, Isabelle Petipas, Rudi Lurz, Paulo Tavares, Mário A Santos, Carlos São-José
Role of bacteriophage SPP1 tail spike protein gp21 on host cell receptor binding and trigger of phage DNA ejection.
Mol Microbiol: 2012, 83(2);289-303
[PubMed:22171743] [WorldCat.org] [DOI] (I p)

Lina Jakutytė, Rudi Lurz, Catarina Baptista, Rut Carballido-Lopez, Carlos São-José, Paulo Tavares, Rimantas Daugelavičius
First steps of bacteriophage SPP1 entry into Bacillus subtilis.
Virology: 2012, 422(2);425-34
[PubMed:22154073] [WorldCat.org] [DOI] (I p)

Lina Jakutytė, Catarina Baptista, Carlos São-José, Rimantas Daugelavičius, Rut Carballido-López, Paulo Tavares
Bacteriophage infection in rod-shaped gram-positive bacteria: evidence for a preferential polar route for phage SPP1 entry in Bacillus subtilis.
J Bacteriol: 2011, 193(18);4893-903
[PubMed:21705600] [WorldCat.org] [DOI] (I p)

Elodie Marchadier, Rut Carballido-López, Sophie Brinster, Céline Fabret, Peggy Mervelet, Philippe Bessières, Marie-Françoise Noirot-Gros, Vincent Fromion, Philippe Noirot
An expanded protein-protein interaction network in Bacillus subtilis reveals a group of hubs: Exploration by an integrative approach.
Proteomics: 2011, 11(15);2981-91
[PubMed:21630458] [WorldCat.org] [DOI] (I p)

Catarina Baptista, Mário A Santos, Carlos São-José
Phage SPP1 reversible adsorption to Bacillus subtilis cell wall teichoic acids accelerates virus recognition of membrane receptor YueB.
J Bacteriol: 2008, 190(14);4989-96
[PubMed:18487323] [WorldCat.org] [DOI] (I p)

Celia Plisson, Helen E White, Isabelle Auzat, Amineh Zafarani, Carlos São-José, Sophie Lhuillier, Paulo Tavares, Elena V Orlova
Structure of bacteriophage SPP1 tail reveals trigger for DNA ejection.
EMBO J: 2007, 26(15);3720-8
[PubMed:17611601] [WorldCat.org] [DOI] (P p)

Carlos São-José, Sophie Lhuillier, Rudi Lurz, Ronald Melki, Jean Lepault, Mário Almeida Santos, Paulo Tavares
The ectodomain of the viral receptor YueB forms a fiber that triggers ejection of bacteriophage SPP1 DNA.
J Biol Chem: 2006, 281(17);11464-70
[PubMed:16481324] [WorldCat.org] [DOI] (P p)

Jean-Christophe Meile, Ling Juan Wu, S Dusko Ehrlich, Jeff Errington, Philippe Noirot
Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory.
Proteomics: 2006, 6(7);2135-46
[PubMed:16479537] [WorldCat.org] [DOI] (P p)

Carlos São-José, Catarina Baptista, Mário A Santos
Bacillus subtilis operon encoding a membrane receptor for bacteriophage SPP1.
J Bacteriol: 2004, 186(24);8337-46
[PubMed:15576783] [WorldCat.org] [DOI] (P p)