HemN

• Description: anaerobic coproporphyrinogen III oxidase
  
  

Gene name	hemN
Synonyms	yqeR
Essential	no
Product	anaerobic coproporphyrinogen III oxidase
Function	heme biosynthesis
Gene expression levels in SubtiExpress: hemN
MW, pI	41 kDa, 5.534
Gene length, protein length	1098 bp, 366 aa
Immediate neighbours	hrcA, lepA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis of cofactors

The gene

Basic information

• Locus tag: BSU25500

Database entries

• BsubCyc: BSU25500

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Coproporphyrinogen-III + 2 S-adenosyl-L-methionine = protoporphyrinogen-IX + 2 CO₂ + 2 L-methionine + 2 5'-deoxyadenosine (according to Swiss-Prot)

• Protein family: anaerobic coproporphyrinogen III oxidase family (according to Swiss-Prot)

• Paralogous protein(s): HemZ

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU25500

• Structure:

• UniProt: P54304

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: lepA-hemN PubMed

• Expression browser: hemN PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information: There is a terminator between lepA and hemN with only little readthrough

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

G Homuth, A Rompf, W Schumann, D Jahn
Transcriptional control of Bacillus subtilis hemN and hemZ.
J Bacteriol: 1999, 181(19);5922-9
[PubMed:10498703] [WorldCat.org] [DOI] (P p)

B Hippler, G Homuth, T Hoffmann, C Hungerer, W Schumann, D Jahn
Characterization of Bacillus subtilis hemN.
J Bacteriol: 1997, 179(22);7181-5
[PubMed:9371469] [WorldCat.org] [DOI] (P p)

G Homuth, M Heinemann, U Zuber, W Schumann
The genes of lepA and hemN form a bicistronic operon in Bacillus subtilis.
Microbiology (Reading): 1996, 142 ( Pt 7);1641-9
[PubMed:8757728] [WorldCat.org] [DOI] (P p)