Nth

• Description: endonuclease III, removes abasic sites during base excision repair
  
  

Gene name	nth
Synonyms	jooB, ypoB
Essential	no
Product	endonuclease III
Function	DNA repair
Gene expression levels in SubtiExpress: nth
MW, pI	24 kDa, 9.277
Gene length, protein length	657 bp, 219 aa
Immediate neighbours	ypoC, dnaD
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA repair/ recombination

The gene

Basic information

• Locus tag: BSU22340

Phenotypes of a mutant

• the mutant is sensititive to exposure to H2O2 PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate (according to Swiss-Prot)

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:
  • activity is stimulated by untwisting of the DNA (by DnaD, Hbs, or YonN) PubMed

• Interactions:

• Localization:

Database entries

• Structure: 1P59 (complex with DNA, Geobacillus stearothermophilus)

• UniProt: P39788

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: dnaD-nth PubMed

• Expression browser: nth PubMed

• Sigma factor:

• Regulation:
  • constitutively expressed PubMed
  • expression is transiently increased after treatment with H2O2 PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Justin S Lenhart, Jeremy W Schroeder, Brian W Walsh, Lyle A Simmons
DNA repair and genome maintenance in Bacillus subtilis.
Microbiol Mol Biol Rev: 2012, 76(3);530-64
[PubMed:22933559] [WorldCat.org] [DOI] (I p)

Original publications

Christopher Collier, Cristina Machón, Geoff S Briggs, Wiep Klaas Smits, Panos Soultanas
Untwisting of the DNA helix stimulates the endonuclease activity of Bacillus subtilis Nth at AP sites.
Nucleic Acids Res: 2012, 40(2);739-50
[PubMed:21954439] [WorldCat.org] [DOI] (I p)

J Christopher Fromme, Gregory L Verdine
Structure of a trapped endonuclease III-DNA covalent intermediate.
EMBO J: 2003, 22(13);3461-71
[PubMed:12840008] [WorldCat.org] [DOI] (P p)