DgcW

• Description: potential diguanylate cyclase and phosphodiesterase
  
  

Gene name	ykoW
Synonyms
Essential	no
Product	potential diguanylate cyclase and phosphodiesterase
Function	unknown
Gene expression levels in SubtiExpress: ykoW
MW, pI	84 kDa, 6.823
Gene length, protein length	2247 bp, 749 aa
Immediate neighbours	ykoV, ykoX
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

membrane proteins, metabolism of signalling nucleotides

The gene

Basic information

• Locus tag: BSU13420

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:
  • contains a central GGDEF domain and a C-terminal EAL domain PubMed
  • aa 290-735 are similar to E. coli CsrD (18% identity, 43% similarity)

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: O34311

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: ykoW PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • GP850 (ykoW::cat), available in Jörg Stülke's lab
  • BP140 (ermC) available in Fabian Commichau's lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

• Antibody:

References

Yun Chen, Yunrong Chai, Jian-hua Guo, Richard Losick
Evidence for cyclic Di-GMP-mediated signaling in Bacillus subtilis.
J Bacteriol: 2012, 194(18);5080-90
[PubMed:22821967] [WorldCat.org] [DOI] (I p)

Kazushi Suzuki, Paul Babitzke, Sidney R Kushner, Tony Romeo
Identification of a novel regulatory protein (CsrD) that targets the global regulatory RNAs CsrB and CsrC for degradation by RNase E.
Genes Dev: 2006, 20(18);2605-17
[PubMed:16980588] [WorldCat.org] [DOI] (P p)

M Y Galperin, T A Gaidenko, A Y Mulkidjanian, M Nakano, C W Price
MHYT, a new integral membrane sensor domain.
FEMS Microbiol Lett: 2001, 205(1);17-23
[PubMed:11728710] [WorldCat.org] [DOI] (P p)