IspA

• Description: intracellular serine protease
  
  

Gene name	ispA
Synonyms
Essential	no
Product	intracellular serine protease
Function	protein degradation
Gene expression levels in SubtiExpress: ispA
MW, pI	33 kDa, 4.522
Gene length, protein length	957 bp, 319 aa
Immediate neighbours	metE, rsbRB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of nitrogen sources other than amino acids, proteolysis

This gene is a member of the following regulons

CodY regulon

The gene

Basic information

• Locus tag: BSU13190

Database entries

• BsubCyc: BSU13190

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: peptidase S8 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU13190

• Structure:

• UniProt: P11018

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ispA (according to DBTBS)

• Expression browser: ispA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed

• Regulatory mechanism:
  • CodY: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Boris R Belitsky, Abraham L Sonenshein
Genome-wide identification of Bacillus subtilis CodY-binding sites at single-nucleotide resolution.
Proc Natl Acad Sci U S A: 2013, 110(17);7026-31
[PubMed:23569278] [WorldCat.org] [DOI] (I p)

Y Koide, A Nakamura, T Uozumi, T Beppu
Cloning and sequencing of the major intracellular serine protease gene of Bacillus subtilis.
J Bacteriol: 1986, 167(1);110-6
[PubMed:3087947] [WorldCat.org] [DOI] (P p)