MscL

• Description: large conductance mechanosensitive channel protein, prevents selective release of cytoplasmic proteins in a hypotonic environment
  
  

Gene name	mscL
Synonyms	ywpC
Essential	no
Product	large conductance mechanosensitive channel protein
Function	resistance to osmotic downshockglycine betaine export
Gene expression levels in SubtiExpress: mscL
Metabolic function and regulation of this protein in SubtiPathways: Stress
MW, pI	14 kDa, 4.649
Gene length, protein length	390 bp, 130 aa
Immediate neighbours	ywpD, ywpB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

coping with hypo-osmotic stress, membrane proteins

The gene

Basic information

• Locus tag: BSU36360

Phenotypes of a mutant

sensitive to osmotic downshock (> 0.5 M) during logarithmic growth PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: mscL family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane PubMed

Database entries

• Structure: 3HZQ (MscL of Staphylococcus aureus) PubMed

• UniProt: P94585

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: mscL PubMed

• Expression browser: mscL PubMed

• Sigma factor:

• Regulation: expressed in logarithmic phase PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • 1A957 ( mscL::spec), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Jan Maarten van Dijl, Groningen, Netherlands

Erhard Bremer, University of Marburg, Germany homepage

References

Reviews

Ian R Booth, Paul Blount
The MscS and MscL families of mechanosensitive channels act as microbial emergency release valves.
J Bacteriol: 2012, 194(18);4802-9
[PubMed:22685280] [WorldCat.org] [DOI] (I p)

Ching Kung, Boris Martinac, Sergei Sukharev
Mechanosensitive channels in microbes.
Annu Rev Microbiol: 2010, 64;313-29
[PubMed:20825352] [WorldCat.org] [DOI] (I p)

Valeria Vásquez, Eduardo Perozo
Structural biology: A channel with a twist.
Nature: 2009, 461(7260);47-9
[PubMed:19727188] [WorldCat.org] [DOI] (I p)

Christopher D Pivetti, Ming-Ren Yen, Samantha Miller, Wolfgang Busch, Yi-Hsiung Tseng, Ian R Booth, Milton H Saier
Two families of mechanosensitive channel proteins.
Microbiol Mol Biol Rev: 2003, 67(1);66-85, table of contents
[PubMed:12626684] [WorldCat.org] [DOI] (P p)