AsnO

• Description: asparagine synthase (glutamine-hydrolysing)
  
  

Gene name	asnO
Synonyms	yisO, yucB
Essential	no
Product	asparagine synthase (glutamine-hydrolysing)
Function	biosynthesis of asparagine
Gene expression levels in SubtiExpress: asnO
Metabolic function and regulation of this protein in SubtiPathways: Asp, Asn
MW, pI	70 kDa, 5.696
Gene length, protein length	1842 bp, 614 aa
Immediate neighbours	yisN, yizA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, sporulation proteins

This gene is a member of the following regulons

SigE regulon

The gene

Basic information

• Locus tag: BSU10790

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + L-aspartate + L-glutamine + H₂O = AMP + diphosphate + L-asparagine + L-glutamate (according to Swiss-Prot)

• Protein family: glutamine amidotransferase type-2 domain (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O05272

• KEGG entry: [3]

• E.C. number: 6.3.5.4

Expression and regulation

• Operon: asnO PubMed

• Expression browser: asnO PubMed

• Sigma factor: SigE PubMed

• Regulation:
  • expressed during sporulation (SigE) PubMed
  • subject to positive regulation by SpoIIID PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Patrick Eichenberger, Masaya Fujita, Shane T Jensen, Erin M Conlon, David Z Rudner, Stephanie T Wang, Caitlin Ferguson, Koki Haga, Tsutomu Sato, Jun S Liu, Richard Losick
The program of gene transcription for a single differentiating cell type during sporulation in Bacillus subtilis.
PLoS Biol: 2004, 2(10);e328
[PubMed:15383836] [WorldCat.org] [DOI] (I p)

K Yoshida, Y Fujita, S D Ehrlich
Three asparagine synthetase genes of Bacillus subtilis.
J Bacteriol: 1999, 181(19);6081-91
[PubMed:10498721] [WorldCat.org] [DOI] (P p)