LipB

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  • Description: extracellular esterase, lipase

Gene name lipB
Synonyms yfiP
Essential no
Product extracellular esterase, lipase
Function lipid degradation
Gene expression levels in SubtiExpress: lipB
MW, pI 22 kDa, 9.677
Gene length, protein length 630 bp, 210 aa
Immediate neighbours padR, yfiQ
Sequences Protein DNA DNA_with_flanks
Genetic context
LipB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
LipB expression.png
























Categories containing this gene/protein

utilization of lipids

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU08350

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s): Lip

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon: lipB (according to DBTBS)
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Thorsten Eggert, Ulf Brockmeier, Melloney J Dröge, Wim J Quax, Karl-Erich Jaeger
Extracellular lipases from Bacillus subtilis: regulation of gene expression and enzyme activity by amino acid supply and external pH.
FEMS Microbiol Lett: 2003, 225(2);319-24
[PubMed:12951259] [WorldCat.org] [DOI] (P p)

T Eggert, G van Pouderoyen, B W Dijkstra, K E Jaeger
Lipolytic enzymes LipA and LipB from Bacillus subtilis differ in regulation of gene expression, biochemical properties, and three-dimensional structure.
FEBS Lett: 2001, 502(3);89-92
[PubMed:11583117] [WorldCat.org] [DOI] (P p)

T Eggert, G Pencreac'h, I Douchet, R Verger, K E Jaeger
A novel extracellular esterase from Bacillus subtilis and its conversion to a monoacylglycerol hydrolase.
Eur J Biochem: 2000, 267(21);6459-69
[PubMed:11029590] [WorldCat.org] [DOI] (P p)

G Kobayashi, J Toida, T Akamatsu, H Yamamoto, T Shida, J Sekiguchi
Accumulation of an artificial cell wall-binding lipase by Bacillus subtilis wprA and/or sigD mutants.
FEMS Microbiol Lett: 2000, 188(2);165-9
[PubMed:10913700] [WorldCat.org] [DOI] (P p)