YxeI

• Description: penicillin acylase
  
  

Gene name	yxeI
Synonyms
Essential	no
Product	penicillin acylase
Function	resistance to penicillin
Gene expression levels in SubtiExpress: yxeI
MW, pI	37 kDa, 5.276
Gene length, protein length	984 bp, 328 aa
Immediate neighbours	yxeJ, yxeH
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

Resistance against toxins/ antibiotics

The gene

Basic information

• Locus tag: BSU39540

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: acylation of penicillin V PubMed

• Protein family: peptidase C59 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:
  • the apparent K(m) values for penicillin V and the synthetic substrate 2-nitro-5-(phenoxyacetamido)-benzoic acid was 40mM and 0.63mM, respectively PubMed

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • homotetramer PubMed

• Localization:

Database entries

• Structure: 2OQC

• UniProt: P54948

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: yxeI-yxeJ PubMed

• Expression browser: yxeI PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Priya Rathinaswamy, Sushama M Gaikwad, C G Suresh, Asmita A Prabhune, James A Brannigan, Guy G Dodson, Archana V Pundle
Purification and characterization of YxeI, a penicillin acylase from Bacillus subtilis.
Int J Biol Macromol: 2012, 50(1);25-30
[PubMed:21978958] [WorldCat.org] [DOI] (I p)

Kyle N Erwin, Shunji Nakano, Peter Zuber
Sulfate-dependent repression of genes that function in organosulfur metabolism in Bacillus subtilis requires Spx.
J Bacteriol: 2005, 187(12);4042-9
[PubMed:15937167] [WorldCat.org] [DOI] (P p)

Ken-Ichi Yoshida, Izumi Ishio, Eishi Nagakawa, Yoshiyuki Yamamoto, Mami Yamamoto, Yasutaro Fujita
Systematic study of gene expression and transcription organization in the gntZ-ywaA region of the Bacillus subtilis genome.
Microbiology (Reading): 2000, 146 ( Pt 3);573-579
[PubMed:10746760] [WorldCat.org] [DOI] (P p)