DacC

• Description: penicillin-binding protein 4A, D-alanyl-D-alanine carboxypeptidase
  
  

Gene name	dacC
Synonyms	pbp
Essential	no
Product	penicillin-binding protein 4A, D-alanyl-D-alanine carboxypeptidase
Function	carboxypeptidase
Gene expression levels in SubtiExpress: dacC
MW, pI	52 kDa, 5.413
Gene length, protein length	1473 bp, 491 aa
Immediate neighbours	ppsA, galM
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall synthesis

This gene is a member of the following regulons

SigH regulon

The gene

Basic information

• Locus tag: BSU18350

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Additional information

• A mutation was found in this gene after evolution under relexed selection for sporulation PubMed

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: cleavage ofD-Ala-D-Ala interpeptide bridges in peptidoglycan PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • may be part of the cell wall biosynthetic complex PubMed

• Localization:

Database entries

• Structure: 1W5D, 2J9P (complex with a peptidoglycan mimetic peptide)

• UniProt: P39844

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: galM-dacC PubMed

• Expression browser: dacC PubMed

• Sigma factor: SigH PubMed, SigB PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Venkatesh V Nemmara, S A Adediran, Kinjal Dave, Colette Duez, R F Pratt
Dual substrate specificity of Bacillus subtilis PBP4a.
Biochemistry: 2013, 52(15);2627-37
[PubMed:23560856] [WorldCat.org] [DOI] (I p)

Venkatesh V Nemmara, Liudmila Dzhekieva, Kumar Subarno Sarkar, S A Adediran, Colette Duez, Robert A Nicholas, R F Pratt
Substrate specificity of low-molecular mass bacterial DD-peptidases.
Biochemistry: 2011, 50(46);10091-101
[PubMed:22029692] [WorldCat.org] [DOI] (I p)

Christopher T Brown, Laura K Fishwick, Binna M Chokshi, Marissa A Cuff, Jay M Jackson, Travis Oglesby, Alison T Rioux, Enrique Rodriguez, Gregory S Stupp, Austin H Trupp, James S Woollcombe-Clarke, Tracy N Wright, William J Zaragoza, Jennifer C Drew, Eric W Triplett, Wayne L Nicholson
Whole-genome sequencing and phenotypic analysis of Bacillus subtilis mutants following evolution under conditions of relaxed selection for sporulation.
Appl Environ Microbiol: 2011, 77(19);6867-77
[PubMed:21821766] [WorldCat.org] [DOI] (I p)

Colette Duez, Astrid Zervosen, Nathalie Teller, Rémy Melkonian, Emmanuel Banzubazé, Fabrice Bouillenne, André Luxen, Jean-Marie Frère
Characterization of the proteins encoded by the Bacillus subtilis yoxA-dacC operon.
FEMS Microbiol Lett: 2009, 300(1);42-7
[PubMed:19758330] [WorldCat.org] [DOI] (I p)

Eric Sauvage, Colette Duez, Raphaël Herman, Frédéric Kerff, Stephanie Petrella, John W Anderson, S A Adediran, R F Pratt, Jean-Marie Frère, Paulette Charlier
Crystal structure of the Bacillus subtilis penicillin-binding protein 4a, and its complex with a peptidoglycan mimetic peptide.
J Mol Biol: 2007, 371(2);528-39
[PubMed:17582436] [WorldCat.org] [DOI] (P p)

Dirk-Jan Scheffers, Laura J F Jones, Jeffery Errington
Several distinct localization patterns for penicillin-binding proteins in Bacillus subtilis.
Mol Microbiol: 2004, 51(3);749-64
[PubMed:14731276] [WorldCat.org] [DOI] (P p)

A Petersohn, J Bernhardt, U Gerth, D Höper, T Koburger, U Völker, M Hecker
Identification of sigma(B)-dependent genes in Bacillus subtilis using a promoter consensus-directed search and oligonucleotide hybridization.
J Bacteriol: 1999, 181(18);5718-24
[PubMed:10482513] [WorldCat.org] [DOI] (P p)

D L Popham, M E Gilmore, P Setlow
Roles of low-molecular-weight penicillin-binding proteins in Bacillus subtilis spore peptidoglycan synthesis and spore properties.
J Bacteriol: 1999, 181(1);126-32
[PubMed:9864321] [WorldCat.org] [DOI] (P p)

L B Pedersen, T Murray, D L Popham, P Setlow
Characterization of dacC, which encodes a new low-molecular-weight penicillin-binding protein in Bacillus subtilis.
J Bacteriol: 1998, 180(18);4967-73
[PubMed:9733705] [WorldCat.org] [DOI] (P p)