YfkJ

• Description: general stress protein, protein tyrosine phosphatase
  
  

Gene name	yfkJ
Synonyms
Essential	no
Product	protein tyrosine phosphatase
Function	survival of ethanol stress
Gene expression levels in SubtiExpress: yfkJ
MW, pI	17 kDa, 4.563
Gene length, protein length	468 bp, 156 aa
Immediate neighbours	yfkK, yfkI
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

protein modification, general stress proteins (controlled by SigB)

This gene is a member of the following regulons

SigB regulon

The gene

Basic information

• Locus tag: BSU07880

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Protein tyrosine phosphate + H₂O = protein tyrosine + phosphate (according to Swiss-Prot)

• Protein family: low molecular weight phosphotyrosine protein phosphatase family (according to Swiss-Prot)

• Paralogous protein(s): YwlE

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O35016

• KEGG entry: [3]

• E.C. number: 3.1.3.48

Expression and regulation

• Operon: yfkJ-yfkI-yfkH (according to DBTBS)

• Expression browser: yfkJ PubMed

• Sigma factor: SigA PubMed, SigB PubMed

• Regulation: induced by stress (SigB) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Lucia Musumeci, Cristina Bongiorni, Lutz Tautz, Robert A Edwards, Andrei Osterman, Marta Perego, Tomas Mustelin, Nunzio Bottini
Low-molecular-weight protein tyrosine phosphatases of Bacillus subtilis.
J Bacteriol: 2005, 187(14);4945-56
[PubMed:15995210] [WorldCat.org] [DOI] (P p)

Ivan Mijakovic, Lucia Musumeci, Lutz Tautz, Dina Petranovic, Robert A Edwards, Peter Ruhdal Jensen, Tomas Mustelin, Josef Deutscher, Nunzio Bottini
In vitro characterization of the Bacillus subtilis protein tyrosine phosphatase YwqE.
J Bacteriol: 2005, 187(10);3384-90
[PubMed:15866923] [WorldCat.org] [DOI] (P p)

Dirk Höper, Uwe Völker, Michael Hecker
Comprehensive characterization of the contribution of individual SigB-dependent general stress genes to stress resistance of Bacillus subtilis.
J Bacteriol: 2005, 187(8);2810-26
[PubMed:15805528] [WorldCat.org] [DOI] (P p)

C W Price, P Fawcett, H Cérémonie, N Su, C K Murphy, P Youngman
Genome-wide analysis of the general stress response in Bacillus subtilis.
Mol Microbiol: 2001, 41(4);757-74
[PubMed:11532142] [WorldCat.org] [DOI] (P p)