GndA

• Description: NADP-dependent phosphogluconate dehydrogenase
  
  

Gene name	gndA
Synonyms	yqjI
Essential	no
Product	NADP-dependent phosphogluconate dehydrogenase
Function	pentose phosphate pathway
Gene expression levels in SubtiExpress: gndA
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	51 kDa, 5.076
Gene length, protein length	1407 bp, 469 aa
Immediate neighbours	zwf, polY1
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

carbon core metabolism, phosphoproteins

The gene

Basic information

• Locus tag: BSU23860

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 6-phospho-D-gluconate + NADP⁺ = D-ribulose 5-phosphate + CO₂ + NADPH (according to Swiss-Prot)

• Protein family: 6-phosphogluconate dehydrogenase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information: Reversible reaction PubMed

• Domains:

• Modification: phosphorylation on (Thr-15 OR Thr-17) PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 2W8Z (from Geobacillus stearothermophilus, 83% identity, 92% similarity) PubMed

• UniProt: P80859

• KEGG entry: [3]

• E.C. number: 1.1.1.44

Additional information

It contains a cysteine on the active site. The enzyme is a dimer. PubMed

Expression and regulation

• Operon: polY1-gndA (according to DBTBS)

• Expression browser: gndA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: GP1514 (gndA::kan), available in Stülke lab

• Expression vector:
  • pGP1777 (for expression, purification in E. coli with N-terminal Strep-tag, in pGP172, available in Stülke lab)
  • pGP1789 (N-terminal Strep-tag, for SPINE, purification from B. subtilis, in pGP1389) (available in Stülke lab)
  • GP1408 (gndA-Strep (spc)) & GP1410 (gndA-Strep (cat)), purification from B. subtilis, for SPINE, available in Stülke lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab

• Antibody:

• FLAG-tag construct:
  • GP1403 (spc, based on pGP1331), available in the Stülke lab
  • GP1408 (kan, resistance cassette exchange in GP1403), available in the Stülke lab

References

Additional publications: PubMed

Scott Cameron, Viviane P Martini, Jorge Iulek, William N Hunter
Geobacillus stearothermophilus 6-phosphogluconate dehydrogenase complexed with 6-phosphogluconate.
Acta Crystallogr Sect F Struct Biol Cryst Commun: 2009, 65(Pt 5);450-4
[PubMed:19407374] [WorldCat.org] [DOI] (I p)

Boris Macek, Ivan Mijakovic, Jesper V Olsen, Florian Gnad, Chanchal Kumar, Peter R Jensen, Matthias Mann
The serine/threonine/tyrosine phosphoproteome of the model bacterium Bacillus subtilis.
Mol Cell Proteomics: 2007, 6(4);697-707
[PubMed:17218307] [WorldCat.org] [DOI] (P p)