PheA

• Description: prephenate dehydratase
  
  

Gene name	pheA
Synonyms
Essential	no
Product	prephenate dehydratase
Function	biosynthesis of phenylalanine
Gene expression levels in SubtiExpress: pheA
Metabolic function and regulation of this protein in SubtiPathways: Phe, Tyr, Trp
MW, pI	31 kDa, 5.886
Gene length, protein length	855 bp, 285 aa
Immediate neighbours	nadR, pheB
Sequences	Protein DNA Advanced_DNA
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed 500px

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

The gene

Basic information

• Locus tag: BSU27900

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Prephenate = phenylpyruvate + H₂O + CO₂ (according to Swiss-Prot)

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity: subject to feedback inhibtion by phenylalanine PubMed

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P21203

• KEGG entry: [3]

• E.C. number: 4.2.1.51

Expression and regulation

• Operon: pheB-pheA PubMed

• Expression browser: pheA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information: subject to feedback inhibtion by phenylalanine PubMed

Biological materials

• Mutant:
  • 1A617 ( pheA::erm), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532] [WorldCat.org] [DOI] (I p)

K Trach, J A Hoch
The Bacillus subtilis spo0B stage 0 sporulation operon encodes an essential GTP-binding protein.
J Bacteriol: 1989, 171(3);1362-71
[PubMed:2537815] [WorldCat.org] [DOI] (P p)

E W Nester, R A Jensen
Control of aromatic acid biosynthesis in Bacillus subtilis: sequenial feedback inhibition.
J Bacteriol: 1966, 91(4);1594-8
[PubMed:4956345] [WorldCat.org] [DOI] (P p)