FlhO

• Description: flagellar basal-body rod protein, required for hook assembly
  
  

Gene name	flhO
Synonyms	flgG, yvyA
Essential	no
Product	flagellar basal-body rod protein
Function	motility and chemotaxis
Gene expression levels in SubtiExpress: flhO
MW, pI	29 kDa, 4.733
Gene length, protein length	810 bp, 270 aa
Immediate neighbours	flhP, mbl
Sequences	Protein DNA Advanced_DNA
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

motility and chemotaxis

This gene is a member of the following regulons

SigD regulon

The gene

Basic information

• Locus tag: BSU36400

Phenotypes of a mutant

• lack of SigD-dependent gene expression (no hag expression), no motility PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: assembly of the flagellar hook PubMed

• Protein family: flagella basal body rod proteins family (according to Swiss-Prot)

• Paralogous protein(s): FlgE, FlhP

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: extracellular (no signal peptide) PubMed

Database entries

• Structure:

• UniProt: P39752

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • usd-spoIIID-mbl-flhO-flhP PubMed
  • flhO-flhP PubMed

• Expression browser: flhO PubMed

• Sigma factor:
  • usd: SigE PubMed
  • flhO: SigD PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

Birgit Voigt, Haike Antelmann, Dirk Albrecht, Armin Ehrenreich, Karl-Heinz Maurer, Stefan Evers, Gerhard Gottschalk, Jan Maarten van Dijl, Thomas Schweder, Michael Hecker
Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis.
J Mol Microbiol Biotechnol: 2009, 16(1-2);53-68
[PubMed:18957862] [WorldCat.org] [DOI] (I p)

Masakuni Serizawa, Hiroki Yamamoto, Hirotake Yamaguchi, Yasutaro Fujita, Kazuo Kobayashi, Naotake Ogasawara, Junichi Sekiguchi
Systematic analysis of SigD-regulated genes in Bacillus subtilis by DNA microarray and Northern blotting analyses.
Gene: 2004, 329;125-36
[PubMed:15033535] [WorldCat.org] [DOI] (P p)

A Decatur, M T McMurry, B N Kunkel, R Losick
Translation of the mRNA for the sporulation gene spoIIID of Bacillus subtilis is dependent upon translation of a small upstream open reading frame.
J Bacteriol: 1997, 179(4);1324-8
[PubMed:9023218] [WorldCat.org] [DOI] (P p)

Y Abhayawardhane, G C Stewart
Bacillus subtilis possesses a second determinant with extensive sequence similarity to the Escherichia coli mreB morphogene.
J Bacteriol: 1995, 177(3);765-73
[PubMed:7836311] [WorldCat.org] [DOI] (P p)