MenE

• Description: o-succinylbenzoate-CoA ligase
  
  

Gene name	menE
Synonyms
Essential	yes PubMed
Product	o-succinylbenzoate-CoA ligase
Function	biosynthesis of menaquinone
Gene expression levels in SubtiExpress: menE
Metabolic function and regulation of this protein in SubtiPathways: Menaquinone
MW, pI	54 kDa, 5.466
Gene length, protein length	1458 bp, 486 aa
Immediate neighbours	menC, menB
Sequences	Protein DNA Advanced_DNA
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis of cofactors, essential genes

The gene

Basic information

• Locus tag: BSU30790

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + 2-succinylbenzoate + CoA = AMP + diphosphate + 2-succinylbenzoyl-CoA (according to Swiss-Prot)

• Protein family: MenE type 1 subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P23971

• KEGG entry: [3]

• E.C. number: 6.2.1.26

Expression and regulation

• Operon:
  • menF-menD-ytxM-menB-menE-menC PubMed
  • menB-menE-menC PubMed
  • menE-menC PubMed

• Expression browser: menE PubMed

• Sigma factor:
  • menF: SigA PubMed
  • menB: SigA PubMed
  • menE: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Alia Lapidus, Nathalie Galleron, Alexei Sorokin, S Dusko Ehrlich
Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region.
Microbiology (Reading): 1997, 143 ( Pt 11);3431-3441
[PubMed:9387221] [WorldCat.org] [DOI] (P p)

X Qin, H W Taber
Transcriptional regulation of the Bacillus subtilis menp1 promoter.
J Bacteriol: 1996, 178(3);705-13
[PubMed:8550504] [WorldCat.org] [DOI] (P p)

J R Driscoll, H W Taber
Sequence organization and regulation of the Bacillus subtilis menBE operon.
J Bacteriol: 1992, 174(15);5063-71
[PubMed:1629163] [WorldCat.org] [DOI] (P p)