AckA

• Description: acetate kinase
  
  

Gene name	ackA
Synonyms
Essential	no
Product	acetate kinase
Function	overflow metabolism
Gene expression levels in SubtiExpress: ackA
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	42 kDa, 5.191
Gene length, protein length	1185 bp, 395 aa
Immediate neighbours	moaB, ytxK
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

ATP synthesis, carbon core metabolism

This gene is a member of the following regulons

CcpA regulon, CodY regulon

The gene

Basic information

• Locus tag: BSU29470

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + acetate = ADP + acetyl phosphate (according to Swiss-Prot)

• Protein family: acetokinase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 2IIR (from Thermotoga maritima, 54% identity, 73% similarity)

• UniProt: P37877

• KEGG entry: [3]

• E.C. number: 2.7.2.1

Expression and regulation

• Operon: ackA PubMed

• Expression browser: ackA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expression activated by glucose (2.5 fold) (CcpA) PubMed
  • activated during growth in the presence of branched chain amino acids (CodY) PubMed

• Regulatory mechanism:
  • CodY: transcription activation PubMed
  • CcpA: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Linc Sonenshein, Tufts University, Boston, MA, USA Homepage

References

Reviews

Cheryl Ingram-Smith, Stephen R Martin, Kerry S Smith
Acetate kinase: not just a bacterial enzyme.
Trends Microbiol: 2006, 14(6);249-53
[PubMed:16678422] [WorldCat.org] [DOI] (P p)

Alan J Wolfe
The acetate switch.
Microbiol Mol Biol Rev: 2005, 69(1);12-50
[PubMed:15755952] [WorldCat.org] [DOI] (P p)