YteR

• Description: galacturonyl hydrolase, catalyses intracellular degradation of disaccharides generated by YesX
  
  

Gene name	yteR
Synonyms
Essential	no
Product	galacturonyl hydrolase, catalyses intracellular degradation of disaccharides generated by YesX
Function	intracellular degradation of disaccharides generated by YesX
MW, pI	42 kDa, 5.87
Gene length, protein length	1119 bp, 373 aa
Immediate neighbours	yteS, yteP
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources

The gene

Basic information

• Locus tag: BSU30120

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: glycosyl hydrolase 105 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 2GH4, 2D8L (complex with D-GlcA-Gal-nc)

• UniProt: O34559

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: yteR PubMed

• Sigma factor:

• Regulation: induced by rhamnogalacturonan PubMed

• Regulatory mechanism:

• Additional information: the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Akihito Ochiai, Takafumi Itoh, Akiko Kawamata, Wataru Hashimoto, Kousaku Murata
Plant cell wall degradation by saprophytic Bacillus subtilis strains: gene clusters responsible for rhamnogalacturonan depolymerization.
Appl Environ Microbiol: 2007, 73(12);3803-13
[PubMed:17449691] [WorldCat.org] [DOI] (P p)

Takafumi Itoh, Akihito Ochiai, Bunzo Mikami, Wataru Hashimoto, Kousaku Murata
Structure of unsaturated rhamnogalacturonyl hydrolase complexed with substrate.
Biochem Biophys Res Commun: 2006, 347(4);1021-9
[PubMed:16870154] [WorldCat.org] [DOI] (P p)

Takafumi Itoh, Akihito Ochiai, Bunzo Mikami, Wataru Hashimoto, Kousaku Murata
A novel glycoside hydrolase family 105: the structure of family 105 unsaturated rhamnogalacturonyl hydrolase complexed with a disaccharide in comparison with family 88 enzyme complexed with the disaccharide.
J Mol Biol: 2006, 360(3);573-85
[PubMed:16781735] [WorldCat.org] [DOI] (P p)

G Hambraeus, C von Wachenfeldt, L Hederstedt
Genome-wide survey of mRNA half-lives in Bacillus subtilis identifies extremely stable mRNAs.
Mol Genet Genomics: 2003, 269(5);706-14
[PubMed:12884008] [WorldCat.org] [DOI] (P p)