YhfT

• Description: similar to long-chain fatty-acid-CoA ligase, involved in surfactin production
  
  

Gene name	yhfT
Synonyms
Essential	no
Product	unknown
Function	unknown
MW, pI	52 kDa, 5.949
Gene length, protein length	1437 bp, 479 aa
Immediate neighbours	yhfS, yhfU
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

poorly characterized/ putative enzymes

The gene

Basic information

• Locus tag: BSU10360

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s): LcfA, LcfB, YngI

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O07619

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: yhfT PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: available in Mohamed Marahiel's lab PubMed

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Femke I Kraas, Verena Helmetag, Melanie Wittmann, Matthias Strieker, Mohamed A Marahiel
Functional dissection of surfactin synthetase initiation module reveals insights into the mechanism of lipoinitiation.
Chem Biol: 2010, 17(8);872-80
[PubMed:20797616] [WorldCat.org] [DOI] (I p)