YraA

• Description: general stress protein, degradation of damaged thiol-containing proteins
  
  

Gene name	yraA
Synonyms
Essential	no
Product	cysteine proteinase
Function	degradation of damaged thiol-containing proteins
MW, pI	18 kDa, 4.729
Gene length, protein length	507 bp, 169 aa
Immediate neighbours	adhA, sacC
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

proteolysis, general stress proteins (controlled by SigB), resistance against oxidative and electrophile stress

This gene is a member of the following regulons

AdhR regulon, SigB regulon, SigM regulon

The gene

Basic information

• Locus tag: BSU27020

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: peptidase C56 family (according to Swiss-Prot)

• Paralogous protein(s): YfkM

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O06006

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • adhA-yraA PubMed
  • yraA

• Sigma factor:
  • yraA: SigB PubMed, SigM PubMed

• Regulation:
  • adhA: induced in the presence of the toxic carbonyls methylglyoxal and formaldehyde (AdhR) PubMed
  • yraA: induced by stress (SigB) PubMed

• Regulatory mechanism:
  • AdhR: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Thi Thu Huyen Nguyen, Warawan Eiamphungporn, Ulrike Mäder, Manuel Liebeke, Michael Lalk, Michael Hecker, John D Helmann, Haike Antelmann
Genome-wide responses to carbonyl electrophiles in Bacillus subtilis: control of the thiol-dependent formaldehyde dehydrogenase AdhA and cysteine proteinase YraA by the MerR-family regulator YraB (AdhR).
Mol Microbiol: 2009, 71(4);876-94
[PubMed:19170879] [WorldCat.org] [DOI] (I p)

Adrian J Jervis, Penny D Thackray, Chris W Houston, Malcolm J Horsburgh, Anne Moir
SigM-responsive genes of Bacillus subtilis and their promoters.
J Bacteriol: 2007, 189(12);4534-8
[PubMed:17434969] [WorldCat.org] [DOI] (P p)

Dirk Höper, Uwe Völker, Michael Hecker
Comprehensive characterization of the contribution of individual SigB-dependent general stress genes to stress resistance of Bacillus subtilis.
J Bacteriol: 2005, 187(8);2810-26
[PubMed:15805528] [WorldCat.org] [DOI] (P p)