MenB

• Description: naphthoate synthase
  
  

Gene name	menB
Synonyms
Essential	yes PubMed
Product	naphthoate synthase
Function	biosynthesis of menaquinone
Metabolic function and regulation of this protein in SubtiPathways: Menaquinone
MW, pI	29 kDa, 5.343
Gene length, protein length	813 bp, 271 aa
Immediate neighbours	menE, ytxM
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

biosynthesis of cofactors, essential genes

The gene

Basic information

• Locus tag: BSU30800

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: o-succinylbenzoyl-CoA = CoA + 1,4-dihydroxy-2-naphthoate (according to Swiss-Prot)

• Protein family: enoyl-CoA hydratase/isomerase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1RJM (from Mycobacterium tuberculosis, 48% identity, 61% similarity)

• UniProt: P23966

• KEGG entry: [3]

• E.C. number: 4.1.3.36

Expression and regulation

• Operon:
  • menF-menD-ytxM-menB-menE-menC PubMed
  • menB-menE-menC PubMed

• Expression browser: menB PubMed

• Sigma factor:
  • menF: SigA PubMed
  • menB: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Alia Lapidus, Nathalie Galleron, Alexei Sorokin, S Dusko Ehrlich
Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region.
Microbiology (Reading): 1997, 143 ( Pt 11);3431-3441
[PubMed:9387221] [WorldCat.org] [DOI] (P p)

X Qin, H W Taber
Transcriptional regulation of the Bacillus subtilis menp1 promoter.
J Bacteriol: 1996, 178(3);705-13
[PubMed:8550504] [WorldCat.org] [DOI] (P p)

J R Driscoll, H W Taber
Sequence organization and regulation of the Bacillus subtilis menBE operon.
J Bacteriol: 1992, 174(15);5063-71
[PubMed:1629163] [WorldCat.org] [DOI] (P p)