NupP

• Description: ABC transporter for guanosine (membrane protein)
  
  

Gene name	nupP
Synonyms	yufP
Essential	no
Product	ABC transporter for guanosine
Function	uptake of guanosine
Interactions involving this protein in SubtInteract: YufP
MW, pI	36 kDa, 9.389
Gene length, protein length	1044 bp, 348 aa
Immediate neighbours	nupO, nupQ
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

ABC transporters, biosynthesis/ acquisition of nucleotides, membrane proteins

This gene is a member of the following regulons

CodY regulon

The gene

Basic information

• Locus tag: BSU31560

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: AraH/rbsC subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: NupO-(NupP-NupQ) PubMed

• Localization: cell membrane PubMed

Database entries

• Structure:

• UniProt: O05254

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: nupN-nupO-nupP-nupQ PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed

• Regulatory mechanism:
  • CodY: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Boris R Belitsky, Abraham L Sonenshein
CodY-mediated regulation of guanosine uptake in Bacillus subtilis.
J Bacteriol: 2011, 193(22);6276-87
[PubMed:21926227] [WorldCat.org] [DOI] (I p)

Virginie Molle, Yoshiko Nakaura, Robert P Shivers, Hirotake Yamaguchi, Richard Losick, Yasutaro Fujita, Abraham L Sonenshein
Additional targets of the Bacillus subtilis global regulator CodY identified by chromatin immunoprecipitation and genome-wide transcript analysis.
J Bacteriol: 2003, 185(6);1911-22
[PubMed:12618455] [WorldCat.org] [DOI] (P p)

Y Quentin, G Fichant, F Denizot
Inventory, assembly and analysis of Bacillus subtilis ABC transport systems.
J Mol Biol: 1999, 287(3);467-84
[PubMed:10092453] [WorldCat.org] [DOI] (P p)