YclQ

• Description: petrobactin (3.4-catecholate siderophore) ABC transporter (binding protein), major component of the secretome
  
  

Gene name	yclQ
Synonyms
Essential	no
Product	petrobactin ABC transporter (binding protein)
Function	iron acquisition
MW, pI	34 kDa, 6.628
Gene length, protein length	951 bp, 317 aa
Immediate neighbours	yclP, ycnB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

ABC transporters, acquisition of iron, iron metabolism, membrane proteins

This gene is a member of the following regulons

Fur regulon

The gene

Basic information

• Locus tag: BSU03830

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: uptake of the siderophore petrobactin PubMed

• Protein family: Fe/B12 periplasmic-binding domain (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: YclP-(YclN-YclO)-YclQ PubMed

• Localization:
  • associated to the membrane (via YclN-YclO) PubMed
  • extracellular (signal peptide) PubMed

Database entries

• Structure: 3GFV

• UniProt: P94421

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: yclN-yclO-yclP-yclQ PubMed

• Sigma factor:

• Regulation:
  • repressed unless the cells enter an iron starvation (Fur) PubMed

• Regulatory mechanism:
  • Fur: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Nikolas Bugdahn, Florian Peuckert, Alexander G Albrecht, Marcus Miethke, Mohamed A Marahiel, Markus Oberthür
Direct identification of a siderophore import protein using synthetic petrobactin ligands.
Angew Chem Int Ed Engl: 2010, 49(52);10210-3
[PubMed:21117054] [WorldCat.org] [DOI] (I p)

Anna M Zawadzka, Youngchang Kim, Natalia Maltseva, Rita Nichiporuk, Yao Fan, Andrzej Joachimiak, Kenneth N Raymond
Characterization of a Bacillus subtilis transporter for petrobactin, an anthrax stealth siderophore.
Proc Natl Acad Sci U S A: 2009, 106(51);21854-9
[PubMed:19955416] [WorldCat.org] [DOI] (I p)

Birgit Voigt, Haike Antelmann, Dirk Albrecht, Armin Ehrenreich, Karl-Heinz Maurer, Stefan Evers, Gerhard Gottschalk, Jan Maarten van Dijl, Thomas Schweder, Michael Hecker
Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis.
J Mol Microbiol Biotechnol: 2009, 16(1-2);53-68
[PubMed:18957862] [WorldCat.org] [DOI] (I p)

Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711] [WorldCat.org] [DOI] (I p)

Noel Baichoo, Tao Wang, Rick Ye, John D Helmann
Global analysis of the Bacillus subtilis Fur regulon and the iron starvation stimulon.
Mol Microbiol: 2002, 45(6);1613-29
[PubMed:12354229] [WorldCat.org] [DOI] (P p)

Y Quentin, G Fichant, F Denizot
Inventory, assembly and analysis of Bacillus subtilis ABC transport systems.
J Mol Biol: 1999, 287(3);467-84
[PubMed:10092453] [WorldCat.org] [DOI] (P p)