PyrG

• Description: CTP synthase (NH3, glutamine)
  
  

Gene name	pyrG
Synonyms	ctrA
Essential	yes PubMed
Product	CTP synthase (NH3, glutamine)
Function	pyrimidine biosynthesis
Metabolic function and regulation of this protein in SubtiPathways: Pyrimidines, Nucleotides (regulation)
MW, pI	59 kDa, 5.16
Gene length, protein length	1605 bp, 535 aa
Immediate neighbours	ywjG, rpoE
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

biosynthesis/ acquisition of nucleotides, essential genes

The gene

Basic information

• Locus tag: BSU37150

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + UTP + NH₃ = ADP + phosphate + CTP (according to Swiss-Prot)

• Protein family: CTP synthase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1VCM (from Thermus thermophilus, 52% identity, 70% similarity) PubMed

• UniProt: P13242

• KEGG entry: [3]

• E.C. number: 6.3.4.2

Expression and regulation

• Operon: pyrG PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by cytidine nucleotides (RNA switch) PubMed

• Regulatory mechanism:
  • RNA switch: transcription (termination in the presence of cytidine nucleotides) PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Charles L Turnbough
Regulation of gene expression by reiterative transcription.
Curr Opin Microbiol: 2011, 14(2);142-7
[PubMed:21334966] [WorldCat.org] [DOI] (I p)

Charles L Turnbough, Robert L Switzer
Regulation of pyrimidine biosynthetic gene expression in bacteria: repression without repressors.
Microbiol Mol Biol Rev: 2008, 72(2);266-300, table of contents
[PubMed:18535147] [WorldCat.org] [DOI] (I p)

Original publications

Irene E Jensen-MacAllister, Qi Meng, Robert L Switzer
Regulation of pyrG expression in Bacillus subtilis: CTP-regulated antitermination and reiterative transcription with pyrG templates in vitro.
Mol Microbiol: 2007, 63(5);1440-52
[PubMed:17302819] [WorldCat.org] [DOI] (P p)

Alexander K W Elsholz, Casper Møller Jørgensen, Robert L Switzer
The number of G residues in the Bacillus subtilis pyrG initially transcribed region governs reiterative transcription-mediated regulation.
J Bacteriol: 2007, 189(5);2176-80
[PubMed:17158658] [WorldCat.org] [DOI] (P p)

Qi Meng, Charles L Turnbough, Robert L Switzer
Attenuation control of pyrG expression in Bacillus subtilis is mediated by CTP-sensitive reiterative transcription.
Proc Natl Acad Sci U S A: 2004, 101(30);10943-8
[PubMed:15252202] [WorldCat.org] [DOI] (P p)

Qi Meng, Robert L Switzer
cis-acting sequences of Bacillus subtilis pyrG mRNA essential for regulation by antitermination.
J Bacteriol: 2002, 184(23);6734-8
[PubMed:12426364] [WorldCat.org] [DOI] (P p)

Q Meng, R L Switzer
Regulation of transcription of the Bacillus subtilis pyrG gene, encoding cytidine triphosphate synthetase.
J Bacteriol: 2001, 183(19);5513-22
[PubMed:11544212] [WorldCat.org] [DOI] (P p)

R J Turner, Y Lu, R L Switzer
Regulation of the Bacillus subtilis pyrimidine biosynthetic (pyr) gene cluster by an autogenous transcriptional attenuation mechanism.
J Bacteriol: 1994, 176(12);3708-22
[PubMed:8206849] [WorldCat.org] [DOI] (P p)

C L Quinn, B T Stephenson, R L Switzer
Functional organization and nucleotide sequence of the Bacillus subtilis pyrimidine biosynthetic operon.
J Biol Chem: 1991, 266(14);9113-27
[PubMed:1709162] [WorldCat.org] (P p)

K Trach, J W Chapman, P Piggot, D LeCoq, J A Hoch
Complete sequence and transcriptional analysis of the spo0F region of the Bacillus subtilis chromosome.
J Bacteriol: 1988, 170(9);4194-208
[PubMed:2457578] [WorldCat.org] [DOI] (P p)