GuaA

• Description: GMP synthase (glutamine-hydrolysing)
  
  

Gene name	guaA
Synonyms	guaB
Essential	no
Product	GMP synthetase (glutamine-hydrolysing)
Function	biosynthesis of GMP
Metabolic function and regulation of this protein in SubtiPathways: Purine synthesis, Nucleotides (regulation)
MW, pI	57 kDa, 4.747
Gene length, protein length	1539 bp, 513 aa
Immediate neighbours	yebA, pbuG
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

biosynthesis/ acquisition of nucleotides

The gene

Basic information

• Locus tag: BSU06360

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + xanthosine 5'-phosphate + L-glutamine + H₂O = AMP + diphosphate + GMP + L-glutamate (according to Swiss-Prot)

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:
  • the enzyme is inhibited by guanosine tetraphosphate (stringent response) PubMed

• Interactions:

• Localization:

Database entries

• Structure: 2YWB (from Thermus thermophilus hb8, 45% identity, 57% similarity)

• UniProt: P29727

• KEGG entry: [3]

• E.C. number: 6.3.5.2

Expression and regulation

• Operon: guaA PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

P Mäntsälä, H Zalkin
Cloning and sequence of Bacillus subtilis purA and guaA, involved in the conversion of IMP to AMP and GMP.
J Bacteriol: 1992, 174(6);1883-90
[PubMed:1312531] [WorldCat.org] [DOI] (P p)

H H Saxild, P Nygaard
Regulation of levels of purine biosynthetic enzymes in Bacillus subtilis: effects of changing purine nucleotide pools.
J Gen Microbiol: 1991, 137(10);2387-94
[PubMed:1722815] [WorldCat.org] [DOI] (P p)

T Mitani, J E Heinze, E Freese
Induction of sporulation in Bacillus subtilis by decoyinine or hadacidin.
Biochem Biophys Res Commun: 1977, 77(3);1118-25
[PubMed:409404] [WorldCat.org] [DOI] (P p)