GlmM

• Description: phosphoglucosamine mutase, required for cell wall synthesis
  
  

Gene name	glmM
Synonyms	ybbT
Essential	yes PubMed
Product	phosphoglucosamine mutase, required for cell wall synthesis
Function	cell wall synthesis
MW, pI	48 kDa, 4.677
Gene length, protein length	1344 bp, 448 aa
Immediate neighbours	ybbR, glmS
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

cell wall synthesis, biosynthesis of cell wall components, essential genes, phosphoproteins

The gene

Basic information

• Locus tag: BSU01770

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Alpha-D-glucosamine 1-phosphate = D-glucosamine 6-phosphate (according to Swiss-Prot)

• Protein family: phosphohexose mutase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylation on Ser-100 PubMed, this is an autophosphorylation PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 3I3W (from Francisella tularensis, 39% identity, 58% similarity)

• UniProt: O34824

• KEGG entry: [2]

• E.C. number: 5.4.2.10

Expression and regulation

• Operon: ybbP-ybbR-glmM-glmS

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:
  • pGP400: (expression in B. subtilis in pBQ200), available in Stülke lab
  • pGP1401: (expression, purification in E. coli with N-terminal His-tag, in pWH844), available in Stülke lab
  • pGP1402: (cloning vector for glmM (GlmM S100A) in E. coli, in pBlueskript KS), available in Stülke lab
  • pGP1403: (expression of GlmM (S100A) in B. subtilis in pBQ200), available in Stülke lab
  • pGP1405: (expression, purification of GlmM (S100A) in E. coli with N-terminal His-tag, in pWH844), available in Stülke lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Sebastian R Schmidl, Katrin Gronau, Nico Pietack, Michael Hecker, Dörte Becher, Jörg Stülke
The phosphoproteome of the minimal bacterium Mycoplasma pneumoniae: analysis of the complete known Ser/Thr kinome suggests the existence of novel kinases.
Mol Cell Proteomics: 2010, 9(6);1228-42
[PubMed:20097688] [WorldCat.org] [DOI] (I p)

Christine Eymann, Dörte Becher, Jörg Bernhardt, Katrin Gronau, Anja Klutzny, Michael Hecker
Dynamics of protein phosphorylation on Ser/Thr/Tyr in Bacillus subtilis.
Proteomics: 2007, 7(19);3509-26
[PubMed:17726680] [WorldCat.org] [DOI] (P p)

Boris Macek, Ivan Mijakovic, Jesper V Olsen, Florian Gnad, Chanchal Kumar, Peter R Jensen, Matthias Mann
The serine/threonine/tyrosine phosphoproteome of the model bacterium Bacillus subtilis.
Mol Cell Proteomics: 2007, 6(4);697-707
[PubMed:17218307] [WorldCat.org] [DOI] (P p)

L Jolly, F Pompeo, J van Heijenoort, F Fassy, D Mengin-Lecreulx
Autophosphorylation of phosphoglucosamine mutase from Escherichia coli.
J Bacteriol: 2000, 182(5);1280-5
[PubMed:10671448] [WorldCat.org] [DOI] (P p)

L Jolly, P Ferrari, D Blanot, J Van Heijenoort, F Fassy, D Mengin-Lecreulx
Reaction mechanism of phosphoglucosamine mutase from Escherichia coli.
Eur J Biochem: 1999, 262(1);202-10
[PubMed:10231382] [WorldCat.org] [DOI] (P p)

D Mengin-Lecreulx, J van Heijenoort
Characterization of the essential gene glmM encoding phosphoglucosamine mutase in Escherichia coli.
J Biol Chem: 1996, 271(1);32-9
[PubMed:8550580] [WorldCat.org] [DOI] (P p)