PepT

• Description: peptidase T (tripeptidase), zinc-dependent
  
  

Gene name	pepT
Synonyms
Essential	no
Product	peptidase T (tripeptidase)
Function	peptide degradation
MW, pI	45 kDa, 4.538
Gene length, protein length	1230 bp, 410 aa
Immediate neighbours	yxjL, yxjJ
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU38920

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Release of the N-terminal residue from a tripeptide (according to Swiss-Prot)

• Protein family: peptidase M20B family (according to Swiss-Prot)

• Paralogous protein(s): YqjE

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 3GBO (from Bacillus cereus (71% identity), to be published)

• UniProt: P55179

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: pepT PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

M H Cha, W M Yong, S M Lee, Y S Lee, I Y Chung
The biochemical and molecular characterization of recombinant Bacillus subtilis tripeptidase (PepT) as a zinc-dependent metalloenzyme.
Mol Cells: 2000, 10(4);423-31
[PubMed:10987140] [WorldCat.org] (P p)

O Schrögel, O Krispin, R Allmansberger
Expression of a pepT homologue from Bacillus subtilis.
FEMS Microbiol Lett: 1996, 145(3);341-8
[PubMed:8978088] [WorldCat.org] [DOI] (P p)