GroES

• Description: chaperonin, universally conserved protein
  
  

Gene name	groES
Synonyms
Essential	yes PubMed
Product	chaperonin
Function	protein folding and re-folding
Metabolic function and regulation of this protein in SubtiPathways: Stress
MW, pI	10 kDa, 4.595
Gene length, protein length	282 bp, 94 aa
Immediate neighbours	ydiL, groEL
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU06020

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

chaperones/ protein folding, heat shock proteins, essential genes, universally conserved proteins

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: groES chaperonin family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylated on Ser-36 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions: GroEL-GroES

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P28599

• KEGG entry: [3]

• E.C. number:

Additional information

universally conserved protein

Expression and regulation

• Operon: groES-groEL PubMed

• Sigma factor: SigA PubMed

• Regulation: induced by heat shock (HrcA) PubMed

• Regulatory mechanism: HrcA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Wolfgang Schumann, Bayreuth University, Germany Homepage

References

Boumediene Soufi, Chanchal Kumar, Florian Gnad, Matthias Mann, Ivan Mijakovic, Boris Macek
Stable isotope labeling by amino acids in cell culture (SILAC) applied to quantitative proteomics of Bacillus subtilis.
J Proteome Res: 2010, 9(7);3638-46
[PubMed:20509597] [WorldCat.org] [DOI] (I p)

A Schulz, W Schumann
hrcA, the first gene of the Bacillus subtilis dnaK operon encodes a negative regulator of class I heat shock genes.
J Bacteriol: 1996, 178(4);1088-93
[PubMed:8576042] [WorldCat.org] [DOI] (P p)

G Yuan, S L Wong
Isolation and characterization of Bacillus subtilis groE regulatory mutants: evidence for orf39 in the dnaK operon as a repressor gene in regulating the expression of both groE and dnaK.
J Bacteriol: 1995, 177(22);6462-8
[PubMed:7592421] [WorldCat.org] [DOI] (P p)

A Schmidt, M Schiesswohl, U Völker, M Hecker, W Schumann
Cloning, sequencing, mapping, and transcriptional analysis of the groESL operon from Bacillus subtilis.
J Bacteriol: 1992, 174(12);3993-9
[PubMed:1350777] [WorldCat.org] [DOI] (P p)

M Li, S L Wong
Cloning and characterization of the groESL operon from Bacillus subtilis.
J Bacteriol: 1992, 174(12);3981-92
[PubMed:1350776] [WorldCat.org] [DOI] (P p)