Pgk

• Description: phosphoglycerate kinase, glycolytic/ gluconeogenic enzyme, universally conserved protein
  
  

Gene name	pgk
Synonyms
Essential	No
Product	phosphoglycerate kinase
Function	enzyme in glycolysis/ gluconeogenesis
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	42,0 kDa, 4.77
Gene length, protein length	1182 bp, 394 amino acids
Immediate neighbours	tpi, gapA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU33930

Phenotypes of a mutant

• no growth on LB medium, the mutant requires glucise and malate for growth
• essential acoording to Kobayashi et al. PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + 3-phospho-D-glycerate = ADP + 1,3-bisphosphoglycerate (according to Swiss-Prot)

• Protein family: phosphoglycerate kinase family (according to Swiss-Prot)
• Paralogous protein(s):

Extended information on the protein

• Kinetic information: Two Substrate Reversible Michaelis-Menten PubMed

• Domains:
  • nucleotide binding domain (ATP) (350–353)
  • 2x substrate binding domain (21–23), (59–62)

• Modification: phosphorylation on Ser-183 AND Thr-299 PubMed, PubMed

• Cofactor(s): Mg2+ or Mn2+ PubMed

• Effectors of protein activity:
  • Inhibited by Co2+, NDP and NMP PubMed

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot), Cytoplasm (Homogeneous) PubMed

Database entries

• Structure: 1PHP (Geobacillus stearothermophilus), Geobacillus stearothermophilus NCBI

• UniProt: P40924

• KEGG entry: [3]

• E.C. number: 2.7.2.3

Expression and regulation

• Operon:
  • cggR-gapA-pgk-tpiA-pgm-eno PubMed
  • pgk-tpiA-pgm-eno PubMed

• Sigma factor:
  • cggR: SigA PubMed
  • pgk: SigA PubMed

• Regulation:
  • expression activated by glucose (3.3 fold) PubMed
  • cggR: induced by glycolytic substrates CggR PubMed
  • pgk: constitutive PubMed

• Regulatory mechanism: transcription repression by CggR PubMed

• Additional information:

Biological materials

• Mutant:
  • GP699 (pgk::cat), available in Stülke lab
  • GP707 (pgk::erm), available in Stülke lab

• Expression vector:
  • pGP1102 (N-terminal His-tag, in pWH844), available in Stülke lab
  • pGP95 (N-terminal Strep-tag, in pGP172), available in Stülke lab
  • pGP91 (N-terminal Strep-tag, for SPINE, expression in B. subtilis, in pGP380), available in Stülke lab
  • pGP1513 (expression in B. subtilis, in pBQ200), available in Stülke lab

• lacZ fusion: pGP514 (in pAC6), a series of promoter deletion variants is also available in pAC6, available in Stülke lab

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab

• Antibody: