AraD

• Description: L-ribulose-phosphate 4-epimerase
  
  

Gene name	araD
Synonyms
Essential	no
Product	L-ribulose-phosphate 4-epimerase
Function	arabinose utilization
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	25 kDa, 5.243
Gene length, protein length	687 bp, 229 aa
Immediate neighbours	araL, araB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU28780

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: L-ribulose 5-phosphate = D-xylulose 5-phosphate (according to Swiss-Prot)

• Protein family: AraD/fucA subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1K0W (from Escherichia coli, 59% identity, 74% similarity) PubMed

• UniProt: P94525

• KEGG entry: [3]

• E.C. number: 5.1.3.4

Expression and regulation

• Operon: araA-araB-araD-araL-araM-araN-araP-araQ-abfA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by glucose (CcpA) PubMed
  • induced by arabinose (AraR) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed
  • AraR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: