MmgB

• Description: 3-hydroxyacyl-CoA dehydrogenase (acetoacetyl-CoA)
  
  

Gene name	mmgB
Synonyms	yqiM
Essential	no
Product	3-hydroxyacyl-CoA dehydrogenase (acetoacetyl-CoA)
Function	mother cell metabolism
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	26 kDa, 6.602
Gene length, protein length	732 bp, 244 aa
Immediate neighbours	mmgC, mmgA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU24160

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

sporulation proteins, poorly characterized/ putative enzymes

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: (S)-3-hydroxybutanoyl-CoA + NADP⁺ = 3-acetoacetyl-CoA + NADPH (according to Swiss-Prot)

• Protein family: 3-hydroxyacyl-CoA dehydrogenase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P45856

• KEGG entry: [3]

• E.C. number: 1.1.1.157

Expression and regulation

• Operon: mmgA-mmgB-mmgC-mmgD-mmgE-yqiQ PubMed

• Sigma factor: SigE PubMed

• Regulation:
  • expressed early during sporulation in the mother cell (SigE) PubMed
  • repressed by glucose (CcpA) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

E M Bryan, B W Beall, C P Moran
A sigma E dependent operon subject to catabolite repression during sporulation in Bacillus subtilis.
J Bacteriol: 1996, 178(16);4778-86
[PubMed:8759838] [WorldCat.org] [DOI] (P p)