YkfC

• Description: D-glutamyl-L-amino acid peptidase
  
  

Gene name	ykfC
Synonyms
Essential	no
Product	D-glutamyl-L-amino acid peptidase
Function	cell wall metabolism
MW, pI	32 kDa, 6.346
Gene length, protein length	888 bp, 296 aa
Immediate neighbours	ykfB, ykfD
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU12990

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: cleaves L-Ala-γ-D-Glu dipeptides from cell wall peptides PubMed

• Protein family: nlpC/p60 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O35010

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Sigma factor:

• Regulation: repressed by CodY PubMed

• Regulatory mechanism: CodY: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Qingping Xu, Polat Abdubek, Tamara Astakhova, Herbert L Axelrod, Constantina Bakolitsa, Xiaohui Cai, Dennis Carlton, Connie Chen, Hsiu Ju Chiu, Michelle Chiu, Thomas Clayton, Debanu Das, Marc C Deller, Lian Duan, Kyle Ellrott, Carol L Farr, Julie Feuerhelm, Joanna C Grant, Anna Grzechnik, Gye Won Han, Lukasz Jaroszewski, Kevin K Jin, Heath E Klock, Mark W Knuth, Piotr Kozbial, S Sri Krishna, Abhinav Kumar, Winnie W Lam, David Marciano, Mitchell D Miller, Andrew T Morse, Edward Nigoghossian, Amanda Nopakun, Linda Okach, Christina Puckett, Ron Reyes, Henry J Tien, Christine B Trame, Henry van den Bedem, Dana Weekes, Tiffany Wooten, Andrew Yeh, Keith O Hodgson, John Wooley, Marc André Elsliger, Ashley M Deacon, Adam Godzik, Scott A Lesley, Ian A Wilson
Structure of the γ-D-glutamyl-L-diamino acid endopeptidase YkfC from Bacillus cereus in complex with L-Ala-γ-D-Glu: insights into substrate recognition by NlpC/P60 cysteine peptidases.
Acta Crystallogr Sect F Struct Biol Cryst Commun: 2010, 66(Pt 10);1354-64
[PubMed:20944232] [WorldCat.org] [DOI] (I p)

Virginie Molle, Yoshiko Nakaura, Robert P Shivers, Hirotake Yamaguchi, Richard Losick, Yasutaro Fujita, Abraham L Sonenshein
Additional targets of the Bacillus subtilis global regulator CodY identified by chromatin immunoprecipitation and genome-wide transcript analysis.
J Bacteriol: 2003, 185(6);1911-22
[PubMed:12618455] [WorldCat.org] [DOI] (P p)