Sandbox

• Description: penicillin-binding protein 5, D-alanyl-D-alanine carboxypeptidase
  
  

Gene name	dacB
Synonyms
Essential	no
Product	penicillin-binding protein 5, D-alanyl-D-alanine carboxypeptidase
Function	carboxypeptidase
MW, pI	42 kDa, 9.725
Gene length, protein length	1146 bp, 382 aa
Immediate neighbours	spmA, ypuI
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU23190

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala (according to Swiss-Prot)

• Protein family: peptidase S11 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• Swiss prot entry: P35150

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: dacB-spmA-spmB

• Sigma factor: SigE Link to a summary

• Regulation: expressed during sporulation in the mother cell Link to a summary

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Ralf Moeller, Peter Setlow, Günther Reitz, Wayne L Nicholson
Roles of small, acid-soluble spore proteins and core water content in survival of Bacillus subtilis spores exposed to environmental solar UV radiation.
Appl Environ Microbiol: 2009, 75(16);5202-8
[PubMed:19542328] [WorldCat.org] [DOI] (I p)

Dirk-Jan Scheffers, Laura J F Jones, Jeffery Errington
Several distinct localization patterns for penicillin-binding proteins in Bacillus subtilis.
Mol Microbiol: 2004, 51(3);749-64
[PubMed:14731276] [WorldCat.org] [DOI] (P p)

D L Popham, J Meador-Parton, C E Costello, P Setlow
Spore peptidoglycan structure in a cwlD dacB double mutant of Bacillus subtilis.
J Bacteriol: 1999, 181(19);6205-9
[PubMed:10498740] [WorldCat.org] [DOI] (P p)

D L Popham, M E Gilmore, P Setlow
Roles of low-molecular-weight penicillin-binding proteins in Bacillus subtilis spore peptidoglycan synthesis and spore properties.
J Bacteriol: 1999, 181(1);126-32
[PubMed:9864321] [WorldCat.org] [DOI] (P p)

D L Popham, B Illades-Aguiar, P Setlow
The Bacillus subtilis dacB gene, encoding penicillin-binding protein 5*, is part of a three-gene operon required for proper spore cortex synthesis and spore core dehydration.
J Bacteriol: 1995, 177(16);4721-9
[PubMed:7642500] [WorldCat.org] [DOI] (P p)

C E Buchanan, M L Ling
Isolation and sequence analysis of dacB, which encodes a sporulation-specific penicillin-binding protein in Bacillus subtilis.
J Bacteriol: 1992, 174(6);1717-25
[PubMed:1548223] [WorldCat.org] [DOI] (P p)