CotW

• Description: spore coat protein (insoluble fraction)
  
  

Gene name	cotW
Synonyms
Essential	no
Product	spore coat protein (insoluble fraction)
Function	resistance of the spore
MW, pI	12 kDa, 9.955
Gene length, protein length	315 bp, 105 aa
Immediate neighbours	cotX, cotV
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU11770

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: CotV-CotW PubMed

• Localization:

Database entries

• Structure:

• Swiss prot entry: Q08310

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: cotV-cotW-cotX PubMed

• Sigma factor: SigK PubMed

• Regulation: repressed by casamino acids PubMed

• Regulatory mechanism: GerE: transcription activation PubMed,

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Daniela Krajcíková, Magda Lukácová, Denisa Müllerová, Simon M Cutting, Imrich Barák
Searching for protein-protein interactions within the Bacillus subtilis spore coat.
J Bacteriol: 2009, 191(10);3212-9
[PubMed:19304857] [WorldCat.org] [DOI] (I p)

Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147] [WorldCat.org] [DOI] (P p)

J Zhang, H Ichikawa, R Halberg, L Kroos, A I Aronson
Regulation of the transcription of a cluster of Bacillus subtilis spore coat genes.
J Mol Biol: 1994, 240(5);405-15
[PubMed:7519271] [WorldCat.org] [DOI] (P p)