Difference between revisions of "Sandbox"

From SubtiWiki
Jump to: navigation, search
Line 1: Line 1:
* '''Description:''' potential sRNA, might interact with [[CsrA]] <br/><br/>
+
* '''Description:''' repressor (MarR-type) of spx, yodC and azoR1 expression, induction in the presence of quinones due to the formation of YodB-Cys6-S-adducts <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''rsmY''
+
|''yodB''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''csrB ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
+
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || potential sRNA
+
|style="background:#ABCDEF;" align="center"| '''Product''' || transcriptional repressor (MarR-type)
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || [[CsrA]]-binding RNA, antagonizing [[CsrA]] regulation
+
|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of quinone detoxification
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || xx,x kDa, y.yy
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 12 kDa, 4.737 
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || aaa bp, bbb amino acids
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 336 bp, 112 aa
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yybO]]'', ''[[yybN]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yodA]]'', ''[[yodC]]''
 
|-
 
|-
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/genE_nucleotide.txt    Gene sequence      (+200bp)  ]'''
+
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13845&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/genE_protein.txt Protein sequence]'''
 
 
|-
 
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:rsmY_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:yodB_context.gif]]
 +
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
 
|}
 
|}
Line 34: Line 34:
  
 
=== Basic information ===
 
=== Basic information ===
+
 
* '''Locus tag:'''
+
* '''Locus tag:''' BSU19540
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
Line 43: Line 43:
 
* '''DBTBS entry:''' no entry
 
* '''DBTBS entry:''' no entry
  
* '''SubtiList entry:'''
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13531]
  
 
=== Additional information===
 
=== Additional information===
  
  
=The RNA=
+
=The protein=
  
 
=== Basic information/ Evolution ===
 
=== Basic information/ Evolution ===
  
* '''Biological activity:'''  
+
* '''Catalyzed reaction/ biological activity:'''  
 +
 
 +
* '''Protein family:''' SunT subfamily (according to Swiss-Prot)
 +
 
 +
* '''Paralogous protein(s):'''
 +
 
 +
=== Extended information on the protein ===
 +
 
 +
* '''Kinetic information:'''
 +
 
 +
* '''Domains:'''
 +
 
 +
* '''Modification:'''
 +
 
 +
* '''Cofactor(s):'''
 +
 
 +
* '''Effectors of protein activity:'''
 +
 
 +
* '''Interactions:'''
 +
 
 +
* '''Localization:'''
 +
 
 +
=== Database entries ===
 +
 
 +
* '''Structure:'''
 +
 
 +
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/O34844 O34844]
  
* '''Interactions:''' [[CsrA]]-[[RsmY]] (hypothetical)
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU19540]
  
* '''Structure:'''
+
* '''E.C. number:'''
  
 
=== Additional information===
 
=== Additional information===
Line 74: Line 100:
 
=Biological materials =
 
=Biological materials =
  
* '''Mutant:''' GP822, aphA3, available in the [[Stülke]] lab
+
* '''Mutant:'''
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''
+
       
 
* '''lacZ fusion:'''
 
* '''lacZ fusion:'''
  

Revision as of 22:05, 12 June 2009

  • Description: repressor (MarR-type) of spx, yodC and azoR1 expression, induction in the presence of quinones due to the formation of YodB-Cys6-S-adducts

Gene name yodB
Synonyms
Essential no
Product transcriptional repressor (MarR-type)
Function regulation of quinone detoxification
MW, pI 12 kDa, 4.737
Gene length, protein length 336 bp, 112 aa
Immediate neighbours yodA, yodC
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YodB context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU19540

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: SunT subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed