LicH

• Description: 6-phospho-beta-glucosidase
  
  

Gene name	licH
Synonyms	celD, celF
Essential	no
Product	6-phospho-beta-glucosidase
Function	lichenan utilization
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	48 kDa, 5.183
Gene length, protein length	1326 bp, 442 aa
Immediate neighbours	ywaA, licA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU38560

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 6-phospho-beta-D-glucosyl-(1,4)-D-glucose + H₂O = D-glucose + D-glucose 6-phosphate (according to Swiss-Prot)

• Protein family: glycosyl hydrolase 4 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1S6Y (Geobacillus stearothermophilus)

• Swiss prot entry: P46320

• KEGG entry: [3]

• E.C. number: 3.2.1.86

Expression and regulation

• Operon: licB-licC-licA-licH PubMed

• Sigma factor: SigA PubMed

• Regulation: repressed by glucose (CcpA) , carbon catabolite repression, induction by oligomeric ß-glucosides PubMed

• Regulatory mechanism: CcpA: transcription repression, catabolite repression: repression by CcpA, induction: transcription activation by the PRD-type regulator LicR PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

S Tobisch, P Glaser, S Krüger, M Hecker
Identification and characterization of a new beta-glucoside utilization system in Bacillus subtilis.
J Bacteriol: 1997, 179(2);496-506
[PubMed:8990303] [WorldCat.org] [DOI] (P p)

1. Tobisch, S., P. Glaser, S. Krüger, and M. Hecker. 1997. Identification and characterization of a new ß-glucoside utilization system in Bacillus subtilis. J. Bacteriol. 179:496-506. PubMed
2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed